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C7559

Sigma-Aldrich

Chloroform

≥99%, PCR Reagent, contains amylenes as stabilizer

Synonym(e):

Methylidyntrichlorid, Trichlormethan

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About This Item

Empirische Formel (Hill-System):
CHCl3
CAS-Nummer:
67-66-3
Molekulargewicht:
119.38
Beilstein:
1731042
EG-Nummer:
200-663-8
MDL-Nummer:
MFCD00000826
UNSPSC-Code:
12191502
PubChem Substanz-ID:
24892970
NACRES:
NA.28

Qualität

PCR Reagent

Qualitätsniveau

200

Dampfdichte

4.1 (vs air)

Dampfdruck

160 mmHg ( 20 °C)

Assay

≥99%

Form

liquid

Enthält

amylenes as stabilizer

Verpackung

vial of 1.5 mL

Methode(n)

PCR: suitable

Farbe

colorless

Brechungsindex

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp (Schmelzpunkt)

−63 °C (lit.)

Dichte

1.492 g/mL at 25 °C (lit.)

Fremdaktivität

DNase, RNase, none detected

SMILES String

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChIKey

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Allgemeine Beschreibung

Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent. Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources.

Anwendung

Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids. It has also been used in RNA extraction/isolation and purification.

Piktogramme

Skull and crossbonesHealth hazard
GHS06,GHS08

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Zielorgane

Central nervous system, Liver,Kidney

Lagerklassenschlüssel

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Protokolle

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D4184)

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Amplification of DNA Using Jumpstart™ Taq DNA Polymerase (D9307)

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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