C4618

Monoclonal Anti-Cathepsin L antibody produced in mouse

clone CPL33/1, purified from hybridoma cell culture

• Synonym(e): Anti-CATL, Anti-CTSL, Anti-MEP
• MDL-Nummer: MFCD01321805
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 200
• Konjugat: unconjugated
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: CPL33/1, monoclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~25 kDa (human cathepsin L); antigen ~42 kDa (human pro cathepsin L)
• Speziesreaktivität: human
• Konzentration: ~2 mg/mL
• Methode(n): immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.1-0.2 μg/mL using total cell extracts of A549 cells
• Isotyp: IgG1
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CTSL1(1514)

Beschreibung

Allgemeine Beschreibung

Anti-Cathepsin L antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human procathepsin L.

Monoclonal Anti-Cathepsin L (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from BALB/c mice immunized with human procathepsin L.

Spezifität

The antibody recognizes the native and denaturated forms of the protein and does not cross react with human cathepsin V. Monoclonal Anti-Cathepsin L specifically recognizes human cathepsin L (∼ 25 kDa) and procathepsin L (∼ 42 kDa). Anti-Cathepsin L antibody epitope resides within amino acids of human cathepsin L (FYKE).

Immunogen

human procathepsin L. The antibody epitope resides within amino acids 258-261 of human cathepsin L (FYKE).

Anwendung

Anti-Cathepsin L antibody has been used for immunoblotting.

Monoclonal Anti-Cathepsin L antibody produced in mouse is suitable for:

• immunohistochemistry
• indirect ELISA
• western blot : 0.1-0.2 μg/mL using total cell extracts of A549 cells

Biochem./physiol. Wirkung

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis.

Inhibition of the enzyme or the proenzyme by low molecular weight inhibitors or by specific antibodies led to a suppression of the invasive capabilities of malignant cells, or a decline in their ability to form tumors in experimental in vivo and in vitro models.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter