C-41210

DetachKit

3 x 125 ml

• Synonym(e): Cell separation kit
• UNSPSC-Code: 12352207
• NACRES: NA.71

Eigenschaften

• Verpackung: pkg of 3 × 125 ml
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

DetachKit-125

Anwendung

The DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells. The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.

Komponenten

Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%. HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.

Qualität

All lots of PromoCell′s DetachKits are subjected to comprehensive quality control tests. Each lot is routinely tested for use with primary human cells, the absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Lagerung und Haltbarkeit

Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label. Thawed solutions can be stored at 4 to 8°C and used for up to 6 weeks.

Subkultur-Routine

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Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids