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Merck

C-23061

Sigma-Aldrich

Skeletal Muscle Differentiation Medium

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

Synonym(e):

Primary Cell Culture Media

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About This Item

UNSPSC-Code:
12352207

Form

liquid

Verpackung

pkg of 500 ml

Methode(n)

cell culture | mammalian: suitable

Versandbedingung

wet ice

Allgemeine Beschreibung

Skeletal Muscle Cell Differentiation Medium (Ready-to-use)

Anwendung

The Skeletal Muscle Cell Differentiation Medium is a serum-free medium developed to induce the fusion of skeletal muscle cells to myotubes with typical multinucleated syncytia. The media are optimized for primary human cells, but can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Recommended for:Human Skeletal Muscle Cells (SkMC)

Qualität

All lots of PromoCell Skeletal Muscle Cell Differentiation Medium is tested for the capacity to fully induce the differentiation of SkMC. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Angaben zur Herstellung

Final supplement concentrations after addition to the basal medium:Insulin (recombinant human): 10 µg / ml

Lagerung und Haltbarkeit

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Subkultur-Routine

Click here for more information.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Artikel

Primary human skeletal muscle cells and culture medium optimized for the in vitro cultivation and differentiation of primary human skeletal muscle cells. Protocols for handling, cell passaging, media, and product use.

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