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Merck

C-12071

Sigma-Aldrich

Human Follicle Dermal Papilla Cells (HFDPC)

500,000 cryopreserved cells

Synonym(e):

HFDPC cells

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About This Item

UNSPSC-Code:
41106514
NACRES:
NA.81

Biologische Quelle

human dermis

Verpackung

pkg of 500,000 cells

Morphologie

( dermal)

Methode(n)

cell culture | mammalian: suitable

Versandbedingung

dry ice

Lagertemp.

−196°C

Allgemeine Beschreibung

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Ursprung der Zelllinie

Skin

Anwendung

Primary Human Follicle Dermal Papilla Cells (HFDPC) are isolated mainly from human dermis of the scalp, originating from the occipital or temple region and are provided in a cryopreserved format. Information on donor hair and skin color is available on request. HFDPC stain positive for alkaline phosphatase.Dermal papillae are embedded in a laminin and collagen IV rich extracellular matrix at the base of the hair follicles. They are essential for the induction and maintenance of hair growth. Since they have androgen receptors, they can be used for in vitro screening of androgen blocking reagents.

Qualität

Rigid quality control tests are performed for each lot of Follicle Dermal Papilla Cells. They are tested for cell morphology, adherence rate, and cell viability. Immunohistochemical tests for significant markers, e.g. alkaline phosphatase, are carried out for each lot. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, and microbial contaminants (fungi, bacteria, and mycoplasma).

Warnhinweis

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subkultur-Routine

Click here for more information.

Sonstige Hinweise

Recommended Plating Density: 5000 - 10000 cells per cm2Passage After Thawing: P2Tested Markers: Alkaline phosphatase positiveGuaranteed population doublings: >10

Empfohlene Produkte

Recommended Primary Cell Culture Media:Link

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Artikel

Primary human follicle dermal papilla cells and optimized culture medium. Protocols for handling, cell passaging, media, and product use.

Primary human follicle dermal papilla cells and optimized culture medium. Protocols for handling, cell passaging, media, and product use.

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