C0618

Anti-Calsequestrin-1

~1.6 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-CASQ1, Aspartactin, CALMITINE, Calsequestrin- skeletal muscle isoform
• MDL-Nummer: MFCD09265355
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen ~60 kDa
• Speziesreaktivität: canine, mouse, human, rat
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Konzentration: ~1.6 mg/mL
• Methode(n): western blot: 0.5-1 μg/mL using extracts of rat skeletal muscle S1 fraction
• UniProt-Hinterlegungsnummer: P31415
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... CASQ1(844) ; mouse ... Casq1(12372)

Beschreibung

Allgemeine Beschreibung

Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca²⁺ release with the help of triadin and junction proteins.

Immunogen

synthetic peptide corresponding to amino acids 66-84 located near the N-terminus of mouse calsequestrin-1, conjugated to KLH. This sequence is identical in human, rat, and dog calsequestrin-1 and highly conserved in bovine (single amino acid substitution). This sequence is not found in calsequestrin-2.

Anwendung

Anti-Calsequestrin-1 antibody produced in rabbit has been used in immunofluorescence.

Anti-Calsequestrin-1 antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using extracts of rat skeletal muscle S1 fraction.

Biochem./physiol. Wirkung

Calsequestrin-1 is a putative auto-antigen associated with eye muscle inflammation in Graves′ disease. Calsequestrin-2 (55 kDa) is found in the SR′s terminal cisternae luminal space of cardiac, slow skeletal muscle cells and may be in platelets. The major Ca²⁺ binding protein in cardiac and skeletal muscle is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which functions as an internal Ca²⁺ store in the lumen of the SR. It helps in controlling the smooth-muscle contraction. Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca²⁺ release with the help of triadin and junction proteins.†

Zielbeschreibung

Calsequestrin (CS also known as CSQ), the major Ca²⁺ binding protein in cardiac and skeletal muscle, is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which function as an internal Ca²⁺ store in the lumen of the SR.1-3 In mammals, two forms of the protein exist, calsequestrin-1 (CASQ-1) and calsequestrin-2 (CASQ-2), which encode the fast-twitch skeletal muscle and cardiac calsequestrin, respectively. Calsequestrin-1 is found in the SRs terminal cisternae luminal space of fast skeletal muscle cells. Calsequestrin-1 is a putative autoantigen associated with eye muscle inflammation in Graves′ disease.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)