A8275
Anti-Kaninchen-IgG-(Gesamtmolekül)-Peroxidase in Ziege hergestellte Antikörper
IgG fraction of antiserum, buffered aqueous solution
Synonym(e):
Goat Anti-Rabbit IgG (whole molecule)–HRP
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About This Item
Empfohlene Produkte
Biologische Quelle
goat
Qualitätsniveau
Konjugat
peroxidase conjugate
Antikörperform
IgG fraction of antiserum
Antikörper-Produkttyp
secondary antibodies
Klon
polyclonal
Form
buffered aqueous solution
Methode(n)
direct ELISA: 1:10,000
Versandbedingung
dry ice
Lagertemp.
−20°C
Posttranslationale Modifikation Target
unmodified
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Allgemeine Beschreibung
IgG, a monomer, is an antibody circulating in blood and can assist in phagocytic destruction of foreign microorganism. It plays an important role in Antibody dependent cell mediated cytotoxicity and is also associated with type II and type III hypersensitivity. Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody can be used in pAb-ELISA. Rabbit anti-IgG antibody coupled with peroxidase (1:2500 dilution in TBS, 0.5% BSA) can be used in western blots. This antibody is also used as detector in indirect ELISA for saxitoxin analysis. Goat anti-rabbit IgG Peroxidase conjugate reacted specifically with all rabbit immunoglobulin.
Immunogen
Purified rabbit IgG.
Anwendung
Es wurden eine Ko-Immunpräzipitation und eine Western-Blot-Analyse von C33A-Zelllysaten durchgeführt, wobei HRP-konjugiertes Anti-Kaninchen-IgG von der Ziege als Sekundärantikörper verwendet wurde.
Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody is used in western blotting, Indirect ELISA, Immunoblotting and Immunodetection.
Immunohistochemistry was performed on frozen sections (10um) of mouse intestine, liver, and spleen using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Prior to incubation with the secondary, sections were treated with a mixture of MeOH/hydrogen peroxide 30% to block endogenouse peroxidases.
Physikalische Form
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.
Angaben zur Herstellung
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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