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Merck

A8229

Sigma-Aldrich

Monoclonal Anti-A2B5 antibody produced in mouse

clone 105, purified immunoglobulin, lyophilized powder

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

105, monoclonal

Form

lyophilized powder

Speziesreaktivität

human, rat, chicken, mouse

Methode(n)

flow cytometry: 2.5 μg using using retinoic acid-differentiated NTera-2 human testicular embryonic carcinoma cell line (per 106 cells)
immunocytochemistry: 8-25 μg/mL using using NTera-2 human cell line

Isotyp

IgM

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

A2B5 is a ganglioside that has recently been used as marker for stem cells.
Monoclonal Anti-A2B5, Clone 105, was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with embryonic chicken retinal cells.
Mouse Monoclonal Anti-A2B5 antibody binds to human, rat, chicken and mouse A2B5.

Immunogen

embryonic chicken retinal cells.

Anwendung

Mouse Monoclonal Anti-A2B5 antibody can be used for flow cytometry (2.5μg) and immunocytochemistry (8-25μg/ml) assays.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

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C M Wen et al.
Journal of fish biology, 90(1), 201-221 (2016-10-13)
In this study, cultures of neural stem-progenitor cells (NSPC) from the brain of green terror cichlid Aequidens rivulatus were established and various NSPCs were demonstrated using immunocytochemistry. All of the NSPCs expressed brain lipid-binding protein, dopamine- and cAMP-regulated neuronal phosphoprotein
Francesca Ruffini et al.
The American journal of pathology, 165(6), 2167-2175 (2004-12-08)
We used expression of the ganglioside A2B5 to isolate putative myelin progenitor cells from adult human central nervous system parenchyma and compared their phenotypic (expression of myelin lineage molecules) and functional (survival, proliferation) properties with mature oligodendrocytes (OLGs) derived from
Nagako Kawashima et al.
Journal of neurochemistry, 111(4), 1031-1041 (2009-09-22)
Sandhoff disease is a progressive neurodegenerative disorder caused by mutations in the HEXB gene which encodes the beta-subunit of N-acetyl-beta-hexosaminidase A and B, resulting in the accumulation of the ganglioside GM2. We isolated astrocytes from the neonatal brain of Sandhoff
C M Wen et al.
Journal of fish biology, 90(1), 201-221 (2016-10-13)
In this study, cultures of neural stem-progenitor cells (NSPC) from the brain of green terror cichlid Aequidens rivulatus were established and various NSPCs were demonstrated using immunocytochemistry. All of the NSPCs expressed brain lipid-binding protein, dopamine- and cAMP-regulated neuronal phosphoprotein
G S Eisenbarth et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(10), 4913-4917 (1979-10-01)
Fusion of spleen cells from a mouse immunized with chicken embryo retina cells with clonal mouse myeloma cells yielded a lymphocyte hybrid cell line that produced antibody that bound to neural tissue such as retina, brain, spinal cord, and dorsal

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