A7419

Avidin–Peroxidase

buffered aqueous solution

• Synonym(e): Avidin–HRP
• MDL-Nummer: MFCD00130587
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: avidin from egg white; enzyme from horseradish
• Qualitätsniveau: 200
• Konjugat: peroxidase conjugate
• Form: buffered aqueous solution
• Methode(n): indirect ELISA: 1:100,000; western blot: 1:400,000-1:800,000 using detecting β-Actin in total cell extract of HeLa cells (5-10 μg/well)
• Versandbedingung: dry ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Avidin is homotetrameric protein (68kDa) obtained from egg whites and binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Anwendung

Avidin-Peroxidase has been used for ELISA applications. The product can also be used for western blot applications at dilutions ranging from 1:400,000 to 1:800,000.

Western blot analysis of proteins in HUVEC cells that had underwent cell surface biotinylation were detected using HRP-conjugated avidin at 33.3 ng/ml.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Angaben zur Herstellung

Affinity purified protein.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable