A5159

Aminopterin

Hybri-Max^™, 50 ×, γ-irradiated, lyophilized powder, BioXtra, suitable for hybridoma

• Synonym(e): (S)-2-{4-[(2,4-Diaminopteridin-6-yl)-methylamino]-benzamido}-pentandisäure, 4-Amino-pteroyl-L-glutaminsäure, 4-Aminofolsäure, erythro-2-Amino-1-phenyl-1-propanol -hydrochlorid
• Empirische Formel (Hill-System): C₁₉H₂₀N₈O₅
• CAS-Nummer: 54-62-6
• Molekulargewicht: 440.41
• Beilstein: 69045
• EG-Nummer: 200-209-9
• UNSPSC-Code: 12352205
• PubChem Substanz-ID: 24890950
• NACRES: NA.75

Eigenschaften

• Biologische Quelle: synthetic
• Qualitätsniveau: 200
• Qualität: Hybri-Max^™
• Sterilität: γ-irradiated
• Produktlinie: BioXtra
• Form: lyophilized powder
• Konzentration: 50 ×
• Methode(n): cell culture | hybridoma: suitable
• Verunreinigungen: endotoxin, tested
• Löslichkeit: 10 mL/vial, clear, red (in TC Medium)
• ε (Extinktionskoeffizient): 24,500 at 282 nm in 0.1 M NaOH at 1 M; 25,700 at 261 nm in 0.1 M NaOH at 1 M; 8,100 at 373 nm in 0.1 M NaOH at 1 M
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• SMILES String: Nc1nc(N)c2nc(CNc3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)cnc2n1
• InChI: 1S/C19H20N8O5/c20-15-14-16(27-19(21)26-15)23-8-11(24-14)7-22-10-3-1-9(2-4-10)17(30)25-12(18(31)32)5-6-13(28)29/h1-4,8,12,22H,5-7H2,(H,25,30)(H,28,29)(H,31,32)(H4,20,21,23,26,27)/t12-/m0/s1
• InChIKey: TVZGACDUOSZQKY-LBPRGKRZSA-N
• Angaben zum Gen: human ... FPGS(2356) ; mouse ... Fpgs(14287)

Beschreibung

Anwendung

Aminopterin concentrate supplement for hybridoma cell culture applications.

Biochem./physiol. Wirkung

Folic acid antagonist. Aminopterin is actively transported into cells by the folate transporter. In the cell, it is converted to a high molecular weight polyglutamate metabolite by folylpolyglutamate synthase that, in turn, binds to dihydrofolate reductase and inhibits its activity. Aminopterin-polyglutamate is degraded intracellularly by γ-glutamyl hydrolase.

Verlinkung

Wirksamer aber auch toxischer als Methotrexat

Rekonstituierung

Reconstitute contents of vial with 10 mL sterile cell culture medium. Stock solution is sufficient to prepare 500 mL medium. Final working concentration: 0.4 μM aminopterin.

Rechtliche Hinweise

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Piktogramme: GHS06,GHS08
• Signalwort: Danger
• H-Sätze: H300 - H315 - H319 - H341 - H360FD
• P-Sätze: P202 - P264 - P270 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 1 Oral - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2
• Lagerklassenschlüssel: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges