A5043

Adenosine Deaminase from bovine spleen

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

• Synonym(e): Adenosin-Aminohydrolase
• CAS-Nummer: 9026-93-1
• EC-Nummer: 3.5.4.4 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00081287
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: bovine spleen
• Qualitätsniveau: 200
• Typ: Type X
• Form: buffered aqueous glycerol solution
• Spezifische Aktivität: ≥130 units/mg protein
• Mol-Gew.: 32.5-33 kDa
• Kationenspuren: NH₄⁺: ≤10 μg/mg protein
• UniProt-Hinterlegungsnummer: P56658
• Fremdaktivität: alkaline phosphatase ≤0.01%; guanase ≤0.01%; nucleoside phosphorylase ≤0.1%
• Lagertemp.: 2-8°C
• Angaben zum Gen: cow ... ADA(280712)

Beschreibung

Allgemeine Beschreibung

Molecular weight: 32.5-33 kDa

pI: 4.85

Anwendung

Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem./physiol. Wirkung

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Einheitendefinition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physikalische Form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Hinweis zur Analyse

Protein determined by biuret.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable