A4108
Aminophenyl-Fluorescein-Lösung
5 mM in DMF, suitable for fluorescence
Synonym(e):
2-[6-(4′-Amino)-phenoxy-3H-xanthen-3-on-9-yl]-benzoesäure-Lösung, APF
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About This Item
Allgemeine Beschreibung
Aminophenyl fluorescein solution, also known as APF, is a novel fluorescent probe. APF exists as a non-fluorescent entity, which converts to its fluorescent form called fluorescein upon oxidation. The excitation length is at 488nm, and the emission spectrum is monitored between 495-600nm and measured using flow cytometry.
Fluorescence intensity increases after reaction with hROS (e.g. hydroxy radical, peroxynitrite and hypochlorite). And, APF is resistant to light-induced autoxidation.
Fluorescence intensity increases after reaction with hROS (e.g. hydroxy radical, peroxynitrite and hypochlorite). And, APF is resistant to light-induced autoxidation.
Anwendung
Fluoreszenzdetektor hoch reaktiver Sauerstoffspezies (HROS). Die Fluoreszenzintensität nimmt nach der Reaktion mit HROS (z.B. Hydroxyradikal, Peroxynitrit und Hypochlorit) zu. Da APF nicht mit Stickstoffmonoxid, Superoxid und Wasserstoffperoxid reagiert, kann es verwendet werden, um differentiell Peroxynitrit nachzuweisen. APF ist beständig gegen Licht-induzierte Autoxidation.
Aminophenyl fluorescein solution is a suitable tool to detect hypobromous acid (HOBr) produced in eosinophils. It is used to detect the halogenating activity of heme peroxidases in leukocytes. APF does not react with nitric oxide, superoxide, and hydrogen peroxide; hence, it can be used to detect peroxynitrite differentially.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B
Lagerklassenschlüssel
3 - Flammable liquids
WGK
WGK 2
Flammpunkt (°F)
135.5 °F - closed cup
Flammpunkt (°C)
57.5 °C - closed cup
Persönliche Schutzausrüstung
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Jörg Flemmig et al.
The Journal of biological chemistry, 287(33), 27913-27923 (2012-06-22)
The specific detection of peroxidase activity in human granulocytes is essential to elucidate their role in innate immune responses, immune regulation, and inflammatory diseases. The halogenating activity of myeloperoxidase in neutrophils can be determined by the novel fluorescent probe aminophenyl
Detection of the halogenating activity of heme peroxidases in leukocytes by aminophenyl fluorescein.
J Flemmig et al.
Free radical research, 49(6), 768-776 (2014-12-24)
The formation of hypochlorous and hypobromous acids by heme peroxidases is a key property of certain immune cells. These products are not only involved in defense against pathogenic microorganisms and in regulation of inflammatory processes, but contribute also to tissue
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