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Merck

A1047

Sigma-Aldrich

Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(e):

Goat Anti-Mouse IgG (γ-chain specific)−AP

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

goat

Rekombinant

expressed in goat

Konjugat

alkaline phosphatase conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

mouse

Methode(n)

direct ELISA: 1:7,000-1:21,000
western blot: suitable

Versandbedingung

wet ice

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibody associates with mouse IgG (gamma-chain specific).

Immunogen

purified mouse IgG

Anwendung

Antibodies to Trypanosoma cruzi were detected in the sera of infected mice by ELISA with an alkaline phosphatase conjugated goat anti-mouse IgG chain specific. Plates were coated with T. cruzi antigen and blocked with 1% BSA/PBS prior to incubation with conjugated antibody.
For detection of IgG antibodies in mouse sera and saliva following S. gordonii infection, ELISA assays were performed using anti-mouse IgG alkaline phosphatase antibody at 1:20000. This antibody did not detect IgA antibodies.
Goat anti-mouse IgG (gamma-chain specific)-alkaline phosphatase antibodies can be used for western blot assays,.

Physikalische Form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin and 15 mM sodium azide

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Letícia Lintomen et al.
British journal of pharmacology, 135(5), 1315-1323 (2002-03-06)
1. We evaluated whether immunization affects bone-marrow responses to indomethacin, because allergenic sensitization and challenge upregulate responses to haemopoietic cytokines (including IL-5-driven eosinopoiesis) in murine bone-marrow, while indomethacin upregulates haemopoiesis and protects bone-marrow from radiation damage. 2. Progenitor (semi-solid) and/or
Monika Wilde et al.
Biotechnology letters, 36(4), 743-749 (2014-01-01)
A comparative analysis of new and established insect cell lines, in regard to process relevant parameters, provide data that can be exploited for designing more robust and effective protein production processes. The baculovirus-insect cell expression system has been efficiently used
J Klingström et al.
Journal of medical virology, 68(4), 581-588 (2002-10-12)
In order to investigate rodent host specificity of European hantaviruses, experimental infection of colonized and wild-trapped rodents was performed. In addition to the natural rodent reservoir, Clethrionomys glareolus, Puumala hantavirus (PUUV) could infect colonized Microtus agrestis and Lemmus sibiricus, but
Dieter Palmberger et al.
Biotechnology journal, 9(9), 1206-1214 (2014-04-23)
The baculovirus/insect cell system has proven to be a very powerful tool for the expression of several therapeutics. Nevertheless, these products sometimes suffer from reduced biological activity and unwanted side effects. Several studies have demonstrated that glycosylation can greatly influence
H J Cools et al.
Applied and environmental microbiology, 76(9), 2866-2872 (2010-03-23)
The recent decrease in the sensitivity of the Western European population of the wheat pathogen Mycosphaerella graminicola to azole fungicides has been associated with the emergence and subsequent spread of mutations in the CYP51 gene, encoding the azole target sterol

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