81349

Poly(2′-Desoxyinosinic-2′-Desoxycytidylsäure) Natriumsalz

free from low molecular weight nucleotides, lyophilized

• Synonym(e): Poly (dI-dC) Natriumsalz
• CAS-Nummer: 118578-37-3
• MDL-Nummer: MFCD00147947
• UNSPSC-Code: 12352106
• NACRES: NA.52

Eigenschaften

• Qualität: for molecular biology; lyophilized
• Qualitätsniveau: 100
• Form: powder
• Mol-Gew.: M_r ~2.5 × 10⁶ (broad molecular weight distribution); M_r ~2500000
• Verpackung: ampule of ≥10 units A₂₆₀
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Poly (2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt is an alternating copolymer that acts as a substrate for DNA methyltransferases.

Anwendung

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid) sodium salt has been used in electrophoretic mobility shift assay (EMSA) of the transcription factor AP-2 Alpha (TFAP2A) and in southwestern blotting of human embryonic kidney cells (HEK293) nuclear extract.

Suitable for

• non-specific blocking agent in EMSA
• evaluation of DNA methyltransferases
• DNA-small molecule or drug interaction studies

Biochem./physiol. Wirkung

Poly(2′-deoxyinosinic-2′-deoxycytidylic acid), poly(dI-dC) is a synthetic DNA substrate. At high salt, Poly(dI-dC) exists in left handed helical conformation and reverts to right-handed form upon decreasing salt. It is used as a non-specific competitor in electrophoretic mobility shift assay (EMSA).

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)