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Merck

64958

Sigma-Aldrich

2-Methoxy-2,4-diphenyl-3(2H)-Furanon

suitable for fluorescence, ≥98.0% (HPLC)

Synonym(e):

2,4-Diphenyl-2-methoxy-3(2H)-furanon, MDPF

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About This Item

Empirische Formel (Hill-System):
C17H14O3
CAS-Nummer:
Molekulargewicht:
266.29
Beilstein:
1288529
MDL-Nummer:
UNSPSC-Code:
12352200
PubChem Substanz-ID:
NACRES:
NA.32

Qualitätsniveau

Assay

≥98.0% (HPLC)

Form

solid

Fluoreszenz

λex 384 nm; λem 472 nm in acetonitrile (after derivatization with hexylamine)

Eignung

suitable for fluorescence

Lagertemp.

2-8°C

SMILES String

COC1(OC=C(C1=O)c2ccccc2)c3ccccc3

InChI

1S/C17H14O3/c1-19-17(14-10-6-3-7-11-14)16(18)15(12-20-17)13-8-4-2-5-9-13/h2-12H,1H3

InChIKey

BLWINLJDTOJSRU-UHFFFAOYSA-N

Anwendung

2-Methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) is used as a fluorescence reagent to derivatize primary and secondary amines on molecules such as proteins. MDPF derivatized molecules can be detected during analytical and separation procedures including chromatography, electrophoresis and zymography.

Sonstige Hinweise

Reagens für die Derivatisierung von primären und sekundären Aminen für die HPLC; es werden stark fluoreszierende Derivate gebildet; Vorsäulenderivatisierung von Aminen; Fluoreszenzmarkierung von Proteinen vor SDS-PAGE

Piktogramme

Exclamation mark

Signalwort

Warning

Gefahreneinstufungen

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

dust mask type N95 (US), Eyeshields, Gloves


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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Pablo Castro-Hartmann et al.
Electrophoresis, 25(15), 2501-2505 (2004-08-10)
The high-energy intermediates generated in the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with H2O2 can excite electronically different fluorophores with a high quantum yield in organic solvents. We have previously applied this peroxyoxalate chemiluminescent reaction to the detection of proteins labeled with
F J Alba et al.
Electrophoresis, 18(11), 1960-1966 (1998-01-07)
We have studied the light emission efficiency of proteins labeled with different fluorescent dyes chemically excited by the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H2O2 reaction. Using this peroxyoxalate chemiluminescence system, the best results were obtained with proteins covalently labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF). Blotted proteins
M Hengstschläger et al.
Cytometry, 14(1), 39-45 (1993-01-01)
Thymidine kinase is a key enzyme for the application of drugs in chemotherapy and for diagnosis. Although of great interest, its regulation during cell cycle and differentiation is difficult to study, as current techniques for isolation of cells in different
J C Stockert et al.
Blood cells, 19(2), 423-430 (1993-01-01)
The fluorogenic reagent MDPF forms highly fluorescent products specifically with primary amino groups (e.g., from lysine). Although widely used in analytical biochemistry, MDPF has infrequently been employed in cytochemical techniques for proteins. In this work, we describe the application of
S. Stein
Pept. Neurobiol., 9-9 (1977)

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