47608

Formaldehyd -Lösung

for molecular biology, BioReagent, ≥36.0% in H₂O (T)

• Synonym(e): Formalin
• Lineare Formel: HCHO
• CAS-Nummer: 50-00-0
• Molekulargewicht: 30.03
• Beilstein: 1209228
• MDL-Nummer: MFCD00003274
• UNSPSC-Code: 12352114
• PubChem Substanz-ID: 329757328
• NACRES: NA.52

Eigenschaften

• Qualität: for molecular biology
• Qualitätsniveau: 100
• Dampfdichte: 1.03 (vs air)
• Dampfdruck: 52 mmHg ( 37 °C)
• Produktlinie: BioReagent
• Form: liquid
• Selbstzündungstemp.: 572 °F
• Enthält: ~10% methanol as stabilizer
• Konzentration: ≥36.0% in H₂O (T); 36.0-38.0% (aldehyde method)
• Methode(n): electrophoresis: suitable
• Verunreinigungen: ≤0.03% free acid (as HCOOH)
• Glührückstand: ≤0.05% (as SO₄)
• pH-Wert: 2.8-4.0
• Dichte: 1.09 g/mL at 20 °C; 1.09 g/mL at 25 °C (lit.)
• Anionenspuren: chloride (Cl^-): ≤10 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• Kationenspuren: Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Na: ≤100 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Zn: ≤1 mg/mL
• λ: 2 M in H₂O
• UV-Absorption: λ: 260 nm A_max: 0.01; λ: 280 nm A_max: 0.01
• Eignung: in accordance for electrophoresis of RNA in agarose gels
• Lagertemp.: room temp
• SMILES String: [H]C([H])=O
• InChI: 1S/CH2O/c1-2/h1H2
• InChIKey: WSFSSNUMVMOOMR-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.

Anwendung

Formaldehyde solution is suitable for:

• denaturation and separation of RNA molecules
• anti-bacterial treatment
• fixation of cells

Formaldehydesolution has been used:

• in the fixation of human spermatogonial stem cell transplantation (SSCT).
• to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.
• in fixing zebra fish larvae for neutrophil staining.

Vorsicht

kann Niederschlag enthalten (Paraformaldehyd)

Prinzip

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Sicherheitshinweise

• Piktogramme: GHS02,GHS06,GHS08,GHS05
• Signalwort: Danger
• H-Sätze: H226,H301 + H311,H314,H317,H330,H335,H341,H350,H370
• P-Sätze: P202 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
• Zielorgane: Eyes,Central nervous system, Respiratory system
• Lagerklassenschlüssel: 3 - Flammable liquids
• WGK: WGK 3
• Flammpunkt (°F): 133.0 °F - closed cup
• Flammpunkt (°C): 56.11 °C - closed cup
• Persönliche Schutzausrüstung: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter