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Sigma-Aldrich

Formaldehyd -Lösung

for molecular biology, BioReagent, ≥36.0% in H2O (T)

Synonym(e):

Formalin

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About This Item

Lineare Formel:
HCHO
CAS-Nummer:
50-00-0
Molekulargewicht:
30.03
Beilstein:
1209228
MDL-Nummer:
MFCD00003274
UNSPSC-Code:
12352114
PubChem Substanz-ID:
329757328
NACRES:
NA.52

Qualität

for molecular biology

Qualitätsniveau

100

Dampfdichte

1.03 (vs air)

Dampfdruck

52 mmHg ( 37 °C)

Produktlinie

BioReagent

Form

liquid

Selbstzündungstemp.

572 °F

Enthält

~10% methanol as stabilizer

Konzentration

≥36.0% in H2O (T)
36.0-38.0% (aldehyde method)

Methode(n)

electrophoresis: suitable

Verunreinigungen

≤0.03% free acid (as HCOOH)

Glührückstand

≤0.05% (as SO4)

pH-Wert

2.8-4.0

Dichte

1.09 g/mL at 20 °C
1.09 g/mL at 25 °C (lit.)

Anionenspuren

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤20 mg/kg

Kationenspuren

Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Na: ≤100 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Zn: ≤1 mg/mL

λ

2 M in H2O

UV-Absorption

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

Eignung

in accordance for electrophoresis of RNA in agarose gels

Lagertemp.

room temp

SMILES String

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

InChIKey

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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Allgemeine Beschreibung

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.

Anwendung

Formaldehyde solution is suitable for:
  • denaturation and separation of RNA molecules
  • anti-bacterial treatment
  • fixation of cells
Formaldehydesolution has been used:
  • in the fixation of human spermatogonial stem cell transplantation (SSCT).
  • to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.
  • in fixing zebra fish larvae for neutrophil staining.

Vorsicht

kann Niederschlag enthalten (Paraformaldehyd)

Prinzip

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

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Zielorgane

Respiratory system

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 3

Flammpunkt (°F)

133.0 °F - closed cup

Flammpunkt (°C)

56.11 °C - closed cup

Persönliche Schutzausrüstung

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Analysenzertifikate (COA)

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