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Merck

406-05F

Sigma-Aldrich

Human Osteoblasts: HOb, fetal

Synonym(e):

Hob cells

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About This Item

UNSPSC-Code:
41106514
NACRES:
NA.81

Biologische Quelle

human bone (normal)

Qualitätsniveau

Verpackung

pkg of 500,000 cells

Hersteller/Markenname

Cell Applications, Inc

Wachstumsmodus

Adherent

Karyotyp

2n = 46

Morphologie

osteoblast

Methode(n)

cell culture | mammalian: suitable

Relevante Krankheit(en)

arthritis; osteoporosis

Versandbedingung

dry ice

Lagertemp.

−196°C

Allgemeine Beschreibung

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

HOb are isolated from fetal or adult human bones. Additionally, osteoblasts from osteoarthritis or rheumatoid arthritis donors are available. Human osteoblasts provide an excellent model system for studies relevant to the skeletal system.

Characterization: Positive for bone mineralization.

Human osteoblasts have been used to:
  • Characterize signaling pathways mediating bone morphogenesis, such as identify human Crossveinless-2 as an inhibitor of BMP (Binnerts, 2009); demonstrate crosstalk between Runx2, Osterix, and NELL-1 (Chen, 2011); identify TWEAK as a cytokine regulating RANTES production, BMP-2-induced differentiation, and RANKL expression in osteoblasts (Ando, 2006)
  • Demonstrate that Kobophenol A enhances proliferation of osteoblasts via activation of the p38 pathway (Kwak, 2013) and clarify the relationship between hypertension and osteoporosis by demonstrating that Ang II activates osteoclasts via RANKL induction (Shimizu, 2008, 2012)
  • Identify adiponectin as an activator of IL-6, IL-8, VEGF and MMPs in endothelial cells and osteoblasts, implicating it in the development of arthritis (Lee, 2014)
  • Show that inhibition of NF-κB prevented the progression of bone loss in periodontitis and promoted the wound healing in bone defects through the inhibition of osteoclasts (Shimizu, 2009)
  • Study active compounds from Uraria crinita for their potential to stimulate bone formation and regeneration (Mao, 2014)
  • Investigate properties of osteosarcoma, including the role of fluid pressure in angiogenesis (Nathan, 2008) and novel oncogenes (Both, 2012); and develop better treatment strategies (Ma, 2011)
  • Develop optimal materials, coatings and drug delivery techniques for orthopedic implants and tissue engineering (Frandsen, 2014; Ni, 2012; Pilia, 2013a, b, 2014; Shiels, 2012; Valente, 2012; Zhang, 2010, 2011)

Ursprung der Zelllinie

Bone

Anwendung

model for skeletal system research, signaling pathways, bone morphogenesis, gene expression, cell activation and proliferation, wound healing, bone formation and regeneration, treatment strategies, tissue engineering, in vitro bone models, mineralization, hormone treatments

Komponenten

Basal Medium containing 10% FBS & 10% DMSO

Angaben zur Herstellung

  • 3rd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

Subkultur-Routine

Please refer to the HOb Culture Protocol.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Gul İpek Gundogan
Cytotechnology, 72(6), 847-854 (2020-10-22)
Only a limited number of techniques are available for assessing the effect of different coating materials on cell adherence to screws. In this study, we describe a simple and inexpensive method for evaluation of cell adhesion on irregular surfaces such

Protokolle

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

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