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Merck

28923

Sigma-Aldrich

Atto 550-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

UNSPSC-Code:
12352108
eCl@ss:
34058011
NACRES:
NA.32

Produktlinie

BioReagent

Qualitätsniveau

Assay

≥90% (HPLC)

Form

film

Hersteller/Markenname

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV-Absorption

λ: 553-559 nm Amax

Eignung

suitable for fluorescence

Lagertemp.

−20°C

Anwendung

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550-Biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Rechtliche Hinweise

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
J Oertel et al.
Journal of cancer research and clinical oncology, 126(8), 425-440 (2000-08-29)
Immunocytochemical investigations of fixed cells are used to enhance diagnostic accuracy in haematology and oncology. The alkaline-phosphatase/anti(alkaline phosphatase) technique, immunoperoxidase and the avidin-biotin technique are the most important methods in immunocytochemistry. Tyramide-enhanced immunostaining is a new powerful technique. Peripheral blood
Immunohistochemical methods for the demonstration of tumor markers.
H Denk
Current topics in pathology. Ergebnisse der Pathologie, 77, 47-69 (1987-01-01)
J Chevalier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(4), 481-491 (1997-04-01)
Biotin was recently applied to detect cellular DNA or RNA. In combination with avidin, streptavidin or antibody, it can be conjugated with fluorescent dye, enzyme, ferritin, or gold. However, emphasis has recently been placed on the false-positive results that are
Robert H Meltzer et al.
Lab on a chip, 11(5), 863-873 (2011-01-21)
Rapid, specific, and sensitive detection of airborne bacteria, viruses, and toxins is critical for biodefense, yet the diverse nature of the threats poses a challenge for integrated surveillance, as each class of pathogens typically requires different detection strategies. Here, we

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