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Atto 565 Maleimid
BioReagent, suitable for fluorescence
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About This Item
Empirische Formel (Hill-System):
C37H37ClN4O10
Molekulargewicht:
733.16
MDL-Nummer:
UNSPSC-Code:
12352111
NACRES:
NA.32
Empfohlene Produkte
Produktlinie
BioReagent
Qualitätsniveau
Assay
>90.0% (HPLC)
Form
powder
Hersteller/Markenname
ATTO-TEC GmbH
Durchlässigkeit
254 nm
565 nm
Fluoreszenz
λex 563 nm; λem 592 nm in 0.1 M phosphate pH 7.0
λ
(ethanol with 0.1% trifluoroacetic acid)
UV-Absorption
λ: 560-566 nm Amax
Eignung
corresponds for coupling to thiols
suitable for fluorescence
Lagertemp.
−20°C
Anwendung
Atto 565 is a new label with high molecular absorption (120.000) and quantum yield (0.9) as well as sufficient stoke′s shift (excitation maximum 563 nm, emission maximum 592 nm). Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Maleimides are well suited for coupling to thiol groups. This is similar to iodacetamides, but maleimides do react more thiol selective. They do not show significant reaction with histidine or methionine. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Maleimides may be used for labeling of amines, which usually requires a higher pH than reaction of maleimides with thiols.
Rechtliche Hinweise
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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