137264

HEPES

EMPROVE^® EVOLVE

• Synonym(e): 4-(2-Hydroxyethyl)-piperazin-1-ethansulfonsäure
• Empirische Formel (Hill-System): C₈H₁₈N₂O₄S
• CAS-Nummer: 7365-45-9
• Molekulargewicht: 238.30
• Beilstein: 883043
• MDL-Nummer: MFCD00006158
• UNSPSC-Code: 12161700

Eigenschaften

• Biologische Quelle: synthetic
• Qualitätsniveau: 400
• Produktlinie: EMPROVE^® EVOLVE
• Form: powder
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: Endotoxin, microbial, and trace metals; tested
• pH-Wert: 4.7-5.3; 6.8-8.2
• Nützlicher pH-Bereich: 6.8-8.2
• pKa (25 °C): 7.5
• pKa: 7.5
• Eignung: suitable for manufacturing use
• Fremdaktivität: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• Lagertemp.: 2-30°C
• SMILES String: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChIKey: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Beschreibung

Anwendung

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.HEPES is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Rechtliche Hinweise

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable