PPB022

Tris buffer

Pre-set, pH 7.0, pHast Pack^™, powder

• Synonym(e): 0.1M Tris
• UNSPSC-Code: 12161700
• NACRES: NA.25

Eigenschaften

• Qualitätsniveau: 100
• Produktlinie: pHast Pack^™
• Form: powder
• Lagerbedingungen: (Tightly closed. Dry)
• Verunreinigungen: ≤5 ppm Heavy Metals (lead)
• Farbe: white
• pH-Wert: 7.0
• Löslichkeit: soluble, clear, colorless
• Kationenspuren: Fe: ≤10 ppm
• Fremdaktivität: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Tris buffer, pH 7.0 is a general buffer that is isotonic and non-toxic in nature due to the presence of tris base. It is suitable for most biochemical and molecular biology applications. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

Anwendung

Tris buffer is used in:

• Western blot
• Protein gel electrophoresis
• cell culture (sterile filter buffer)
• As a buffer component

Leistungsmerkmale und Vorteile

• Fast, ready-to-use buffer
• No need to measure and adjust the pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Verpackung

Foil pouches

Rekonstituierung

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 7.0 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

Lagerung und Haltbarkeit

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Sonstige Hinweise

For additional information on our range of Biochemicals, please complete this form.

Rechtliche Hinweise

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1