PPB021

SSPE Buffer

pH 7.4, pHast Pack^™, powder

• Synonym(e): 1X SSPE Hybridization buffer, 1X SSPE buffer, SSPE Hybridization buffer, Saline-Sodium Phosphate-EDTA Buffer
• UNSPSC-Code: 41105319
• NACRES: NA.25

Eigenschaften

• Produktlinie: pHast Pack^™
• Qualitätsniveau: 100
• Form: powder
• pH-Wert: 7.4

Beschreibung

Anwendung

Saline-Sodium Phosphate-EDTA is used as a hybridization buffer in Northern and Southern blotting. It is also used as a wash buffer in DNA-microarray technique.

Leistungsmerkmale und Vorteile

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Save time and minimize effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Verpackung

Foil pouches

Rekonstituierung

Contents of one pouch, when dissolved in 1000 mL of ultrapure water, will yield a 1× solution containing 150 mM NaCl, 10 mM NaH₂PO₄, 1 mM EDTA, pH 7.4 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Lagerung und Haltbarkeit

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Sonstige Hinweise

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Rechtliche Hinweise

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1