PPB015

Tris-Glycine Buffer

pH 8.3, pHast Pack^™, powder

• Synonym(e): 1X TG buffer, TG, TG Buffer, Tris Glycine running buffer, Tris Glycine transfer buffer, Tris glycine
• UNSPSC-Code: 41105319
• NACRES: NA.25

Eigenschaften

• Produktlinie: pHast Pack^™
• Qualitätsniveau: 100
• Form: powder
• Lagerbedingungen: (Tightly closed. Dry)
• Verunreinigungen: ≤5 ppm Heavy Metals (lead)
• Farbe: white to off-white
• pH-Wert: 8.3
• Löslichkeit: soluble, clear, colorless to faintly yellow
• Kationenspuren: Fe: <10 ppm
• Fremdaktivität: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• Lagertemp.: room temp

Beschreibung

Allgemeine Beschreibung

Tris-Glycine buffer is primarily used as a running buffer and to prepare transfer buffer in western blot analysis with the addition of methanol. This buffer can separate proteins by their size. It has a pH of 8.3.

Anwendung

Suitable for use as:

• Transfer buffer component in western blot
• Running buffer in native gel electrophoresis for protein separation
• Running buffer in gradient polyacrylamide gel electrophoresis (PAGE)

Leistungsmerkmale und Vorteile

• Ready-to-use buffer
• No measuring and pH adjusting needed
• Saves time and minimizes the effort
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Verpackung

Foil pouches

Rekonstituierung

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1x solution containing 25 mM Tris, 192 mM Glycine, pH 8.3 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Lagerung und Haltbarkeit

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Sonstige Hinweise

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Rechtliche Hinweise

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1