E1903

Ethidium-Homodimer I -Lösung

≥95% purity (HPLC), liquid

• Synonym(e): EtDi, EthD-1, Phenanthridinium, 5,5′-[1,2-ethandiylbis-(imino-3,1-propandiyl)]-bis-(3, 8-diamino-6-phenyl)-,dichlorid,dihydrochlorid
• Empirische Formel (Hill-System): C₄₆H₅₀Cl₄N₈
• Molekulargewicht: 856.75
• EG-Nummer: 200-664-3
• MDL-Nummer: MFCD00210948
• UNSPSC-Code: 12171500
• PubChem Substanz-ID: 24894444
• NACRES: NA.47

Eigenschaften

• product name: Ethidium-Homodimer I -Lösung, ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO
• Qualitätsniveau: 100
• Assay: ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)
• Form: liquid
• Konzentration: 2 mM in DMSO
• Methode(n): titration: suitable
• Farbe: red
• ε (Extinktionskoeffizient): ≥9000 at 490-496 nm in water
• Anwendung(en): diagnostic assay manufacturing; hematology; histology
• Lagertemp.: −20°C
• SMILES String: Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
• InChI: 1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H
• InChIKey: ZKAJUKABUUJRLB-UHFFFAOYSA-N

Beschreibung

Anwendung

Färbt ssDNA, dsDNA, RNA, Oligonucleotide und Triplex-DNA-Strukturen.

Für DNS-Detektion in Lösung.

Ethidium homodimer I solution has been used to check membrane integrity in sperms.

Biochem./physiol. Wirkung

Ein Molekül EthD-1 bindet nicht-selektiv vier Basenpaare in dsDNA.

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): 185.0 °F - closed cup
• Flammpunkt (°C): 85 °C - closed cup
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)