C5070

CHAPS-Hydrat

BioXtra, ≥98% (TLC)

• Synonym(e): 3-[(3-Cholamidopropyl)dimethylammonio]-1-propansulfonat Hydrat, CHAPS
• Empirische Formel (Hill-System): C₃₂H₅₈N₂O₇S · xH₂O
• CAS-Nummer: 331717-45-4
• Molekulargewicht: 614.88 (anhydrous basis)
• MDL-Nummer: MFCD00012116
• UNSPSC-Code: 12161900
• PubChem Substanz-ID: 24892750
• NACRES: NA.21

Eigenschaften

• Beschreibung: zwitterionic
• Qualitätsniveau: 400
• Produktlinie: BioXtra
• Assay: ≥98% (TLC)
• Form: powder
• Mol-Gew.: 614.88 g/mol (anhydrous basis)
• Methode(n): protein purification: suitable; protein quantification: suitable
• Verunreinigungen: ≤0.001% Phosphorus (P); ≤0.1% Insoluble matter; <0.04% DMF
• Glührückstand: ≤0.1%
• mp (Schmelzpunkt): 157 °C (dec.) (lit.)
• Löslichkeit: H₂O: 0.1 M, clear, colorless
• Anionenspuren: chloride (Cl^-): ≤0.05%
• Kationenspuren: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• SMILES String: O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
• InChIKey: SJCUTFKCLFLIFE-JWTJKVBLSA-N

Beschreibung

Allgemeine Beschreibung

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Anwendung

• 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
• 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
• HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.

Sicherheitshinweise

• Piktogramme: GHS07
• Signalwort: Warning
• H-Sätze: H302
• P-Sätze: P264 - P270 - P301 + P312 - P501
• Gefahreneinstufungen: Acute Tox. 4 Oral
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable