76704

mTSB-Bouillon mit Novobiocin

NutriSelect^® Plus, novobiocin 0.02 g/L, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC)

• Synonym(e): Modifizierte Trypton-Soja-Bouillon – dehydriertes Kulturmedium, Modifizierte Trypton-Soja Bouillon mit Novobiocin
• UNSPSC-Code: 41171607
• NACRES: NA.85

Eigenschaften

• Agentur: according to ISO 16654:2001
• Qualitätsniveau: 200
• Sterilität: non-sterile
• Form: powder
• Haltbarkeit: limited, expiry date on the label
• Zusammensetzung: bile salts No. 3, 1.5 g/L ; di-potassium hydrogen phosphate, 4.0 g/L ; D(+)-glucose, 2.5 g/L ; novobiocin, 0.02 g/L ; peptone from casein, 17.0 g/L ; peptone from soymeal, 3.0 g/L ; sodium chloride, 5.0 g/L
• Hersteller/Markenname: NutriSelect^® Plus
• Verpackung: pkg of -500 g
• Lagerbedingungen: (Dry place, tightly sealed containers)
• Methode(n): microbiological culture: suitable
• Farbe: faint brown
• pH-Endwert: 7.3±0.2 (25 °C)
• Anwendung(en): agriculture; food and beverages; microbiology; pathogen testing
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Eignung: selective for Escherichia coli; selective for coliforms; selective for enterovirulent E. coli (EEC)

Beschreibung

Allgemeine Beschreibung

mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.

Anwendung

Zur selektiven Anreicherung von enterohämorrhagischen E. coli (EHEC) in Nahrungsmitteln.Das Medium wird, gemäss der USDA-FSIS Methode, zur Isolierung und Identifikation von E. coli 0157:H7 in Fleisch eingesetzt.

Angaben zur Herstellung

Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.

Fußnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Rechtliche Hinweise

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)