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Merck

51976

Sigma-Aldrich

Ethyl alcohol, pure

≥99.8%, for molecular biology, (absolute alcohol, without additive, A15 o1), BioUltra

Synonym(e):

Ethylalkohol

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

Lineare Formel:
CH3CH2OH
CAS-Nummer:
Molekulargewicht:
46.07
Beilstein:
1718733
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12191601
PubChem Substanz-ID:
NACRES:
NA.25

product name

Ethanol, BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)

Qualität

for molecular biology
(absolute alcohol, without additive, A15 o1)

Qualitätsniveau

Dampfdichte

1.59 (vs air)

Dampfdruck

44.6 mmHg ( 20 °C)

Produktlinie

BioUltra

Assay

≥99.8%

Form

liquid

Selbstzündungstemp.

683 °F

Expl.-Gr.

19 %, 60 °F

Verunreinigungen

DNases, none detected
KMnO4-reducing substances, in accordance
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
substances darkened by H2SO4, in accordance
≤0.0004% free alkali (as NH3)
≤0.001% aldehyde (as CH3CHO)
≤0.001% formaldehyde (as HCHO)
≤0.001% ketone (as CH3COCH3)
≤0.002% free acid (as CH3COOH)
≤0.1% methanol (CH3OH)
≤0.2% water

Abdampfrückstand

≤0.001%

Brechungsindex

n20/D 1.3600 (lit.)
n20/D 1.362

bp

78 °C (lit.)

mp (Schmelzpunkt)

−114 °C (lit.)

Löslichkeit

H2O: miscible
organic solvents: miscible

Dichte

0.789 g/mL at 25 °C (lit.)

Kationenspuren

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.1 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES String

CCO

λ

neat

UV-Absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.03

Eignung

suitable for molecular biology

Anwendung(en)

life science and biopharma

Format

neat

InChI

1S/C2H6O/c1-2-3/h3H,2H2,1H3

InChIKey

LFQSCWFLJHTTHZ-UHFFFAOYSA-N

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Allgemeine Beschreibung

Ethanol, also known as Ethyl Alcohol, is a volatile primary alcohol with a straight-chain structure. It is frequently employed as a biochemical reagent, effectively denaturing proteins from bacteria, fungi, and viruses. Widely utilized in cell biology, molecular biology, and biochemical research, this DNase, RNase, NICKase, and protease-free form of ethanol plays a crucial role in purifying and precipitating biomolecules like nucleic acids (DNA & RNA) and proteins. Additionally, it finds application in histology for preparing staining and destaining reagents, as well as in the dehydration of tissues before embedding.

Anwendung

Ethanol has been used:
  • in vehicle-treated controls to perform mouse experiments
  • in viability assay along with crystal violet to stain human and mouse high-grade serous ovarian cancer (HGSOC) cells after stimulation
  • to prepare 17β-estradiol (E2) to use in cell culture experiments
  • in cell cycle analysis for fixing cells drop-wise

Biochem./physiol. Wirkung

Ethanol is a widely used organic chemical in industry and consumer goods. This aliphatic alcohol is mainly used in industry as a solvent and as an intermediate in the production of other chemicals.

Leistungsmerkmale und Vorteile

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

Sonstige Hinweise

For additional information on our range of Biochemicals, please complete this form.
Sales restrictions may apply. Suitable for the precipitation of nucleic acids

Fußnote

1Der Vertrieb und der Einsatz von Ethylalkohol in der Schweiz erfordert eine Lizenz der Eidgenössischen Alkoholverwaltung

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Piktogramme

FlameExclamation mark

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Eye Irrit. 2 - Flam. Liq. 2

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 1

Flammpunkt (°F)

55.4 °F - closed cup

Flammpunkt (°C)

13 °C - closed cup

Persönliche Schutzausrüstung

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Die Dokumentenbibliothek aufrufen

G M Huang et al.
Analytical biochemistry, 223(1), 35-38 (1994-11-15)
Genome projects require a high-throughput method for DNA template preparation, which traditional protocols can rarely provide. We introduce here a new protocol for plasmid and cosmid DNA preparation based on alkaline denaturation in a 96-well format. The essential improvement made
M G Mahoney et al.
The Journal of clinical investigation, 103(4), 461-468 (1999-02-18)
Patients with pemphigus foliaceus (PF) have blisters on skin, but not mucous membranes, whereas patients with pemphigus vulgaris (PV) develop blisters on mucous membranes and/or skin. PF and PV blisters are due to loss of keratinocyte cell-cell adhesion in the
D M Lovinger et al.
Science (New York, N.Y.), 243(4899), 1721-1724 (1989-03-31)
The ion current induced by the glutamate receptor agonist N-methyl-D-aspartate (NMDA) in voltage-clamped hippocampal neurons was inhibited by ethanol (EtOH). Inhibition increased in a concentration-dependent manner over the range 5 to 50 mM, a range that also produces intoxication. The
Preparation and properties of serum and plasma proteins; crystallization of serum albumins from ethanol water mixtures.
E J COHN et al.
Journal of the American Chemical Society, 69(7), 1753-1761 (1947-07-01)
Y Gavrieli et al.
The Journal of cell biology, 119(3), 493-501 (1992-11-01)
Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown

Artikel

Substances are said to be miscible in one another if they dissolve to form a uniform solution. Bookmark or download our miscibility table for common lab solvents.

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