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Merck

11218611910

Roche

DNA Molecular Weight Marker VI, DIG-labeled

greener alternative

solution, pkg of 500 μL (10 μg/ml)

Synonym(e):

DNA marker

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About This Item

UNSPSC-Code:
41105335

Form

solution

Qualitätsniveau

Verpackung

pkg of 500 μL (10 μg/ml)

Hersteller/Markenname

Roche

Grünere Alternativprodukt-Eigenschaften

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Grünere Alternativprodukt-Kategorie

Lagertemp.

−20°C

Allgemeine Beschreibung

DNA Molecular Weight Marker VI, DIG-labeled is a fragment mixture prepared by cleavage of pBR328 DNA and pBR328 DNA with restriction endonucleases Bgl I and Hinf I respectively. The size of the fragment ranges from 0.15 to 2.1 kbp.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Anwendung

DNA Molecular Weight Marker VI, DIG-labeled, has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection. It has also been used as a size standard for amplified DNA.

Komponenten

Ready-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.

Sequenz

The mixture contains 12 fragments wit the following base pair lengths: 154/154, 220, 234/234, 298/298, 394, 453, 517, 653, 1033, 1230, 1766, and 2176 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Sonstige Hinweise

12 fragments: 154/154; 220; 234/234; 298/298; 394; 453; 517; 653; 1,033; 1,230; 1,766; 2,176 bp
Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
For life science research only. Not for use in diagnostic procedures.

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

nwg

Flammpunkt (°F)

No data available

Flammpunkt (°C)

No data available


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R Ferretti et al.
Applied and environmental microbiology, 67(2), 977-978 (2001-02-07)
A PCR-based method for the detection of Salmonella spp. in food was developed. The method, set up on typical salami from the Italian region of Marche, is sensitive and specific and shows excellent correlation with the conventional method of reference
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PLoS currents, 6, doi:10-doi:10 (2014-03-13)
Many human diseases are associated with the abnormal expansion of unstable trinucleotide repeat sequences. The mechanisms of trinucleotide repeat size mutation have not been fully dissected, and their understanding must be grounded on the detailed analysis of repeat size distributions

Artikel

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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