PC05L

Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

lyophilized, Calbiochem^®

• UNSPSC-Code: 12352203

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: lyophilized
• Enthält nicht: preservative
• Speziesreaktivität: rat, human, mouse
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Isotyp: IgG
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FOS(2353)

Beschreibung

Allgemeine Beschreibung

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~50-62 kDa c-Fos protein.

Recognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.

The proto-oncogene fos has been implicated in cell growth, differentiation and development. fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.

This Anti-c-Fos (Ab-2) (4-17) Rabbit pAb is validated for use in Frozen Sections, WB, IF, IP, Paraffin Sections, Gel Shift, for the detection of c-Fos (Ab-2) (4-17).

Immunogen

Human

a synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos

Anwendung

Frozen Sections (5-10 µg/ml)

Immunoblotting (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg per reaction)

Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)

Gel Shift (see comments and application references)

Free-floating Sections (not recommended)

Warnhinweis

Toxicity: Standard Handling (A)

Physikalische Form

Lyophilized from PBS, a volatile buffer, 100 µg BSA.

Rekonstituierung

Resuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.

Hinweis zur Analyse

Positive Control
Hep G2 or NIH 3T3 cells

Sonstige Hinweise

DeTogni, P., et al. 1988. Cell Biol.8, 2251.
Rouscher III, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res.49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell51, 513.
Muller, R. 1986. Biochim. Biophys. Acta823, 207.
Verma, I. 1986. Trends Genet.2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433

For gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable