NHPMHMAG-45K
MILLIPLEX® Non-Human Primate Metabolic Magnetic Bead Panel - Metabolism Multiplex Assay
The analytes available for this multiplex kit are: Amylin (Active), C-Peptide, Ghrelin (Active), GIP, Glucagon Like Peptide-1, Glucagon, IL-6, Insulin, Leptin, MCP-1, PP, PYY.
About This Item
Empfohlene Produkte
Qualitätsniveau
Speziesreaktivität
nonhuman primates
Hersteller/Markenname
Milliplex®
assay range
accuracy: 102%
(Ghrelin (Active))
accuracy: 91%
(Insulin)
accuracy: 93%
(GLP-1)
accuracy: 94%
(PP)
accuracy: 98%
(MCP-1)
accuracy: 99%
(Leptin)
sensitivity: 0.6-65.1 pg/mL
standard curve range: 13.7-10,000 pg/mL
(Ghrelin (Active), GLP-1, Glucagon, IL-6, MCP-1, PP, PYY)
standard curve range: 137.2-100,000 pg/mL
(Insulin, Leptin)
standard curve range: 2.7-2,000 pg/mL
(GIP)
standard curve range: 27.4-20,000 pg/mL
(Amylin)
standard curve range: 68.6-50,000 pg/mL
(C-Peptide)
inter-assay cv: <15%
intra-assay cv: <10%
Methode(n)
multiplexing: suitable
Nachweisverfahren
fluorometric (Luminex xMAP)
Versandbedingung
wet ice
Allgemeine Beschreibung
MILLIPLEX® Non-Human Primate Metabolic Panel is a 12-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue/cell lysate and culture supernatant samples: Amylin (active), C-Peptide, Ghrelin (active), GIP (total), GLP-1 (active), Glucagon, IL-6, Insulin, Leptin, MCP-1/CCL2, Pancreatic Polypeptide (PP), and PYY (total). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
Spezifität
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Anwendung
- Analytes: Amylin (Active), C-Peptide, Ghrelin (Active), GIP (Total), Glucagon Like Peptide-1 (GLP-1) (Active), Glucagon, IL-6, Insulin, Leptin, MCP-1 (CCL2), Pancreatic Polypeptide (PP), PYY (Total)
- NOTE: When measuring specific analytes, protease inhibitors should be added to blood samples upon collection: DPPIV inhibitor for GLP-1; Protease Inhibitor Cocktail for Amylin; Aprotinin for Glucagon; Serine Protease Inhibitor for Ghrelin (Active)
- Recommended Sample Type: Non-human primate serum, plasma, cell/tissue culture supernatants and lysates.
- Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; cell/tissue culture samples may require dilution with an appropriate control medium.
- Assay Run Time: Overnight (18-20 hours) at 2-8°C.
- Research Category: Metabolism
- Research Category: Metabolic Diseases, Obesity
Leistungsmerkmale und Vorteile
Verpackung
Lagerung und Haltbarkeit
Rechtliche Hinweise
Haftungsausschluss
Analysenzertifikate (COA)
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Verwandter Inhalt
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
Learn the benefits of using biomarker detection multiplex immunoassays for laboratory, companion, and agricultural animal research and explore MILLIPLEX® multiplex assays for veterinary medicine and animal health research.
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