MSHMAG-21K
MILLIPLEX® Multi-Species Hormone Magnetic Bead Panel
The Multi-Species Hormone Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 steroid/thyroid biomarkers in human serum, plasma and cell culture samples.
Synonym(e):
luminex multiple species thyroid reproductive stress endocrine metabolic hormones multiplex assay, millipore multiple species metabolism hormones multiplex kit, multi species hormone immunoassay panel
About This Item
Empfohlene Produkte
Qualitätsniveau
Speziesreaktivität
human, canine
Hersteller/Markenname
Milliplex®
assay range
accuracy: 105%
(Canine Samples: T3)
accuracy: 80%
(Canine Samples: Estradiol)
accuracy: 85%
(Human Samples: Progesterone)
accuracy: 87%
(Canine Samples: T4)
sensitivity: 0.01 ng/mL
(MinDC+2SD; Estradiol)
sensitivity: 0.12 ng/mL
(MinDC+2SD; T3)
sensitivity: 0.25 ng/mL
(MinDC+2SD; Progesterone)
sensitivity: 0.33 ng/mL
(MinDC+2SD; Cortisol)
sensitivity: 0.51 ng/mL
(MinDC+2SD; T4)
standard curve range: 0.014-10 ng/mL
(Estradiol)
standard curve range: 0.07-50 ng/mL
(T3)
standard curve range: 0.14-100 ng/mL
(Progesterone)
standard curve range: 0.27-200 ng/mL
(Cortisol)
standard curve range: 0.34-250 ng/mL
(T4)
inter-assay cv: <10%
intra-assay cv: <10%
(Cortisol)
inter-assay cv: <15%
intra-assay cv: <10%
(Estradiol)
inter-assay cv: <15%
intra-assay cv: <10%
(Progesterone)
inter-assay cv: <15%
intra-assay cv: <10%
(T3)
inter-assay cv: <15%
intra-assay cv: <10%
(T4)
Methode(n)
multiplexing: suitable
Nachweisverfahren
fluorometric (Luminex xMAP)
Versandbedingung
ambient
Allgemeine Beschreibung
The MILLIPLEX® Multi-Species Hormone Panel is a 5-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and cell/tissue culture samples: Cortisol, Estradiol, Progesterone, T3 and T4. This multi-species kit may be used to detect these analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. The kit has been verified with human and canine samples. The amount of cross-reactivity to other species has been confirmed, but not tested to the same level of verification as was used for human and canine. All analytes in this kit are run in a competitive assay format. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Endocrine
Spezifität
This multi-species kit may be used to detect these 5 analytes in human, dog, rat, mouse, cynomolgous monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was fully validated with human and canine samples only and the amount of cross-reactivity to other species tested has not been determined.
Approximately 10% cross-reactivity to Cortisol is observed in the Progesterone assay and approximately 5% cross-reactivity to T3 is observed in the T4 assay. No other significant cross-reactivity is observed within the panel or to other similar analytes.
Anwendung
- Analytes: Cortisol, Estradiol, Progesterone, T3, T4
- NOTE: This multi-species kit may be used to detect these analytes in human, dog, rat, mouse, cynomolgus monkey, rhesus monkey, horse, rabbit, cat, guinea pig, pig and hamster. However, the kit was validated with human and canine samples only and the amount of cross-reactivity to other species tested, has not been determined.
- NOTE: This assay requires an extraction step for serum and plasma samples (instructions are provided in the kit protocol).
- NOTE: This is a competitive immunoassay
- Recommended Sample Type: Serum, plasma, or tissue/cell culture supernatants or lysates from multiple species
- Recommended Sample Dilution: 25 μL per well of undiluted extracted serum or plasma. Tissue/cell culture samples may be run neat or diluted in an appropriate control media.
- Assay Run Time: Overnight (16-20 hours) at 2-8°C.
- Research Category: Metabolism
- Research Category: Endocrine
Leistungsmerkmale und Vorteile
Verpackung
Verlinkung
Lagerung und Haltbarkeit
Sonstige Hinweise
Rechtliche Hinweise
Haftungsausschluss
Signalwort
Danger
Gefahreneinstufungen
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Zielorgane
Respiratory Tract
Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 3
Analysenzertifikate (COA)
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Verwandter Inhalt
The newer xMAP® Sheath Fluid PLUS formulation contains 3 modes of microbial action to eliminate potential environmental microbial resistance. See how the preparations compare in this performance comparison of the previous xMAP® Sheath Fluid to the new xMAP® Sheath Fluid PLUS formulation in MILLIPLEX® multiplex assays.
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
Learn the benefits of using biomarker detection multiplex immunoassays for laboratory, companion, and agricultural animal research and explore MILLIPLEX® multiplex assays for veterinary medicine and animal health research.
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