MABT219

Anti-Actin Antibody, clone JLA20

clone JLA20, from mouse

• Synonym(e): Actin, alpha skeletal muscle, Alpha-actin-1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: JLA20, monoclonal
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): chicken (immunogen homology)
• Methode(n): immunofluorescence: suitable; western blot: suitable
• Isotyp: IgMκ
• NCBI-Hinterlegungsnummer: NP_001026234
• UniProt-Hinterlegungsnummer: P68139
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ACTA1(58)

Beschreibung

Allgemeine Beschreibung

Actin is an abundantly expressed in eukaryotic cells and it is the core component of the cytoskeleton. It exists in three isoforms: alpha actin, which is found in muscle tissue and forms fibrous actin that facilitates muscle contractility; and beta and gamma actin which are found in non-muscle cells and facilitate cell motility. Actin exists initially as globular actin (G-actin) which is converted to filamentous actin (F-actin) upon assembly. The formation of F-actin is a complex multistep process that is tightly regulated by signaling from the actin-binding proteins (ABPs) and Rho family GTPases. Filamentous actin is crosslinked with other actin polymers to form a complex cytoskeletal network which is required for exo- and phagocytosis, vesicular trafficking, cell migration, and gene regulation, among other cellular processes.

Anwendung

Detect Actin using this Anti-Actin Antibody, clone JLA20 validated for use in Western Blotting, Immunofluorescence.

Immunofluorescence Analysis: A representative lot was used by an independent laboratory in NRK cells. (Cao, L.G., et al. (1993). The Journal of Cell Biology. 123(1):173-181.)

Western Blot Analysis: A representative lot was used by an independent laboratory in Me4405 melanoma cell lysate. (Thomas, W.D., et al. (2000). Journal of Immunology. 165:5612–5620.)

Qualität

Evaluated by Western Blot in SK-BR3 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Actin on 10 µg of SK-BR3 cell lysate.

Zielbeschreibung

~42 kDa observed

Verlinkung

Replaces: 04-1040

Physikalische Form

Format: Purified

Hinweis zur Analyse

Control
SK-BR3 cell lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable