MABS1896

Anti-RNF123 Antibody, clone 2C10.1

clone 2C10.1, from mouse

• Synonym(e): E3 ubiquitin-protein ligase RNF123, EC 2.3.2.27, Kip1 ubiquitination-promoting complex protein 1, RING finger protein 123, RING-type E3 ubiquitin transferase RNF123
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 2C10.1, monoclonal
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25 μL
• Methode(n): western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_071347
• UniProt-Hinterlegungsnummer: Q5XPI4
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... RNF123(63891)

Beschreibung

Allgemeine Beschreibung

E3 ubiquitin-protein ligase RNF123 (UniProt: Q5XPI4; also known as EC 2.3.2.27, Kip1 ubiquitination-promoting complex protein 1, RING finger protein 123, RING-type E3 ubiquitin transferase RNF123) is encoded by the RNF123 (also known as KPC1, PF1477) gene (Gene ID: 63891) in human.RNF123 serves as a catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B/p27 during the cell cycle G1 phase of the cell cycle. It contains a ring-type zinc finger domain (aa 1254-1292). Two isoforms of RNF123 have been reported that are produced by alternative splicing. RNF123 is also reported to serve as a negative regulator of RIG-I and MDA5. Overexpression of RNF123 is shown to inhibit IFN-beta production triggered by Sendai virus (SeV) and encephalomyocarditis picornavirus (EMCV) and knockdown of endogenous RNF123 can potentiate IFN-beta production triggered by SeV and EMCV. RNF123 is shown to interact with the N-terminal CARD domains of RIG-I/MDA5 and compete with the downstream adaptor VISA/MAVS/IPS-1/Cardif for RIG-I/MDA5 CARD binding. (Ref.: Wang, S., et al. (2016). EMBO Rep. 17(8):1155-68).

Spezifität

Clone 2C10.1 specifically detects RING-type E3 ubiquitin transferase RNF123 in human cells. It targets an epitope within 92 amino acids from the N-terminal region.

Immunogen

GST/His-tagged recombinant fragment corresponding to 92 amino acids from the N-terminal region of human RING-type E3 ubiquitin transferase RNF123.

Anwendung

Anti-RNF123, clone 2C10.1, Cat. No. MABS1896, is a mouse monoclonal antibody that detects RING-type E3 ubiquitin transferase RNF123 and has been tested for use in Western Blotting.

Qualität

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RING-type E3 ubiquitin transferase RNF123 in HeLa cell lysate.

Zielbeschreibung

~150 kDa observed; 148.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Format: Purified

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable