MABN691

Anti-GPI Antibody, clone 1B7D7

ascites fluid, clone 1B7D7, from mouse

• Synonym(e): Glucose-6-phosphate isomerase, Autocrine motility factor, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, Sperm antigen 36
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: ascites fluid
• Antikörper-Produkttyp: primary antibodies
• Klon: 1B7D7, monoclonal
• Speziesreaktivität: rat, human
• Methode(n): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG1
• UniProt-Hinterlegungsnummer: P06744
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NLK(51701)

Beschreibung

Allgemeine Beschreibung

Glucose-6-phosphate isomerase (GPI) alternatively known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI) or Sperm antigen 36 (SA-36) and encoded by the gene name GPI is major enzyme in glycolysis and gluconeogenesis. Glucose-6-phosphate isomerase rearranges glucose-6-phosphate (from the phosphorylation of glucose via hexokinases) into fructose-6-phosphate (F6P) as the second step in glycolysis. Interestingly however, Glucose-6-phosphate isomerase plays an entirely different sort of role outside of the cell than when it is inside being part of glycolysis. When secreted, Glucose-6-phosphate isomerase acts as an autocrine motility factor for various cancers and is critical for metastasis, while when secreted in nervous tissue it acts as a neurotrophic factor for sensory and spinal neurons and in lymphocytes it acts as a lymphokine upon T cell ligand binding and stimulates B cells to secrete immunoglobulin. Mutations in Glucose-6-phosphate isomerase are the second most frequent cause of inherited glycolytic-enzymopathy in humans. This autosomal recessive disorder is characterized by anon-spherocytic anemia of variable severity which can present with neuromuscular dysfunctions defined by muscle weakness and mental retardation. EMD-Millipore’s Anti-Glucose-6-phosphate isomerase monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HepG2, SMMC-7721 and rat liver tissue and in paraffin embedded immunohistochemistry on human kidney and fluorescent immunocytochemistry on L-O2 cells in culture.

Immunogen

Purified recombinant fragment of human GPI expressed in E. Coli.

Anwendung

Anti-GPI Antibody, clone 1B7D7 is a highly specific mouse monoclonal antibody, that targets Glucose-6-phosphate isomerase & has been tested in western blotting, IHC & Immunofluorescence.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in human kidney tissue.

Immunofluorescent Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in L-02 cells.

Optimal working dilutions must be determined by end user.

Qualität

Evaluated by Western Blotting in HepG2, SMMC-7721, and rat liver lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPI in HepG2, SMMC-7721, and rat liver lysates.

Zielbeschreibung

~56 kDa observed. Uncharacterized bands may appear in some lysate(s).

Hinweis zur Analyse

Control
HepG2, SMMC-7721, and rat liver lysates

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable