MABN1785

Anti-CD166 (ALCAM) Antibody, clone TAG-1A3

clone TAG-1A3, from mouse

• Synonym(e): Activated leukocyte cell adhesion molecule, CD166 antigen
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: TAG-1A3, monoclonal
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25 μL
• Methode(n): flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_001618
• UniProt-Hinterlegungsnummer: Q13740
• Versandbedingung: ambient
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... ALCAM(214)

Beschreibung

Allgemeine Beschreibung

CD166 antigen (UniProt: Q13740; also known as Activated leukocyte cell adhesion molecule, CD166) is encoded by the ALCAM (also known as MEMD) gene (Gene ID: 214) in human. CD166 is a cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6 and homotypic cell-cell contacts. It also promotes T-cell activation and proliferation via its interactions with CD6 and is shown to be involved in the formation and maturation of the immunological synapse via its interactions with CD6. CD166 mediates attachment of dendritic cells onto endothelial cells via homotypic interaction and inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions. It has an extracellular (aa 28-527), transmembrane (aa 528-549), and a cytoplasmic (aa 550-583) domain. CD166 is expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. It is also detected on hematopoietic stem cells derived from umbilical cord blood, on lymph vessel endothelial cells, skin and tonsil, and on peripheral blood monocytes. Lower levels of CD166 are detected in spleen, placenta, and liver. CD166 is also detected in highly metastasizing melanoma cell lines.

Spezifität

Clone TAG-1A3 specifically detects CD166 in human.

Immunogen

Corneal endothelial cells (hCENC) isolated from donated human cadaves and immunized with monophosphoryl-lipid A trehalose dicorynomycolate (MPLCTDM) adjuvant.

Anwendung

Research Category
Neurowissenschaft

Anti-CD166 (ALCAM) Antibody, clone TAG-1A3, Cat. No. MABN1785, is a highly specific mouse monoclonal antibody that targets CD166 and has been tested in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Flow Cytometry Analysis: A representative lot detected CD166 (ALCAM) in Flow Cytometry applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).

Immunocytochemistry Analysis: A representative lot detected CD166 (ALCAM) in Immunocytochmistry applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).

Immunoprecipitation Analysis: A representative lot detected CD166 (ALCAM) in Immunoprecipitation applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).

Flow Cytometry Analysis: A represetative lot detected CD166 (ALCAM) in human embryonic stem cells, human corneal stromal fibroblast (hCSF) cells, immortalized human corneal endothelial cells (B4G12), and human endothelial cells from donors (2) (Courtesy of Dr. Vanessa Ding, Ph.D. and Dr. Andre Choo, Ph.D, BTI, Singapore).

Immunofluorescence Analysis: A representative lot detected CD166 (ALCAM) in Immunofluorescence applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).

Western Blotting Analysis: A representative lot detected CD166 (ALCAM) in Western Blotting applications (Ding, Y., et. al. (2014). MAbs. 6(6):1439-52).

Qualität

Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be human IgG1 .

Zielbeschreibung

~80 kDa observed; 65.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1