MABF317

Anti-CD3ε (mouse) Antibody, PE, clone 145-2C11

clone 145-2C11, from hamster(Armenian), PE

• Synonym(e): T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: hamster (Armenian)
• Konjugat: PE
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 145-2C11, monoclonal
• Speziesreaktivität: mouse
• Methode(n): activity assay: suitable; flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG
• UniProt-Hinterlegungsnummer: P22646
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Cd3E(12501)

Beschreibung

Allgemeine Beschreibung

CD3, also known as T3, is a member of the Ig and the ITAM superfamilies. The CD3 complex is composed of six polypeptides with usually four different transmembrane CD3 chains, gamma, delta, epsilon and zeta. CD3 is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The CD3 antigen is present in early thymocytes, and has been considered the best all-around T cell marker. The positive staining of this marker may represent a sign of early commitment to the T cell lineage. In cortical thymocytes, during early stages of maturation, the CD3 antigen is predominantly present in the cell cytoplasm. In medullary thymocyles, the CD3 antigen is predominantly detected on the cell surface. The majority of T cell neoplasms also expresses CD3, but it is absent from non-T-cell lymphoid malignancies. CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.

Anwendung

Anti-CD3ε (mouse) Antibody, PE, clone 145-2C11 is a highly specific armenian hamster monoclonal antibody, that targets CD3ε & has been tested in Flow Cytometry, IHC, Activity Assay, IP & western blotting.

Qualität

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Flow Cytometry Analysis: 0.25 μg of this antibody detected CD3ε in one million C57BL/6 mouse splenocytes.

Zielbeschreibung

21 kDa calculated

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2