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MABF2119

Sigma-Aldrich

Anti-CD318 (CD6L) Antibody, clone 3A11

clone 3A11, from mouse

Synonym(e):

CUB domain-containing protein 1, Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with src kinases, CD6L

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

Biologische Quelle

mouse

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

3A11, monoclonal

Speziesreaktivität

human

Verpackung

antibody small pack of 25 μg

Methode(n)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1κ

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... CDCP1(64866)

Allgemeine Beschreibung

CUB domain-containing protein 1 (UniProt: Q9H5V8; also known as Membrane glycoprotein gp140, Subtractive immunization M plus HEp3-associated 135 kDa protein, SIMA135, Transmembrane and associated with Src kinases, CD318, CD6L) is encoded by the CDCP1 (also known as TRASK) gene (Gene ID:64866) in human. CD318 is a single-pass membrane protein (isoform 1) that serves as a ligand for CD6. It is highly expressed in mitotic cells and has low expression during interphase. It is detected in high levels in skeletal muscle and colon tissue and in lower levels in kidney, placenta, lungs, and small intestine. Its levels are shown to be up-regulated in several human cancer tissues. It is also expressed in high levels in synovial tissues where it participates in CD6-dependent adhesion of T cells to synovial fibroblasts. Presence of CD318 on cancer cells is associated with their aggressiveness and metastatic potential. Preincubation of cancer cells with clone 3a11 is shown to enhance killing of these cells and inhibits their survival. CD318 is shown to be chemoattractive to T cells and levels of soluble CD318 are increased in the synovial fluid from patients with rheumatoid arthritis and juvenile inflammatory arthritis. CD318 is synthesized with a signal peptide (aa 1-29), which is cleaved off in the mature form. It has an extracellular domain (aa 30-667), a transmembrane domain (aa 668-688), and a cytoplasmic domain (aa 689-836). Three isoforms of CD318 have been described that are produced by alternative splicing. (Ref.: Ruth, JH., et al. (2021). JCI Insight. doi: 10.1172/jci.insight.145662. Enyindah-Asonye, G., et al. (2017). Proc. Natl. Acad. Sci. USA 114(33); E6912-E6921).

Spezifität

Clone 3A11 specifically detects CD318 in human cells. It targets an epitope with in the extracellular domain.

Immunogen

Epitope: extracellular domain
HBL-100 human mammary gland epithelial cells.

Anwendung

Research Category
Entzündung & Immunologie
Anti-CD318 (CD6L), clone 3A11, Cat. No. MABF2119, is a mouse monoclonal antibody that detects CD318 (CD6L) and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD318 (CD6L) in Immunoprecipitation applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected CD318 (CD6L) in Immunohistochemistry applications (Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).

Western Blotting Analysis: A representative lot detected CD318 (CD6L) in Western Blotting applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).

Flow Cytometry Analysis: A representative lot detected CD318 (CD6L) in Flow Cytometry applications (Saifullah, M.K., et. al. (2004). J Immunol. 173(10):6125-33; Enyindah-Asonye, G., et. al. (2017). Proc Natl Acad Sci U S A. 114(33):E6912-E6921).

Qualität

Evaluated by Immunocytochemistry in HaCaT epidermal keratinocyte cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected CD318 (CD6L) in HaCaT epidermal keratinocyte cells.

Zielbeschreibung

92.93 kDa calculated.

Physikalische Form

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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