MABF2071

Anti-PLVAP Antibody, clone MECA-32

clone MECA-32, from rat

• Synonym(e): Plasmalemma vesicle-associated protein, MECA-32 antigen, Plasmalemma vesicle protein 1, PV-1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: MECA-32, monoclonal
• Speziesreaktivität: mouse
• Speziesreaktivität (Voraussage durch Homologie): human (based on 100% sequence homology)
• Verpackung: antibody small pack of 25 μg
• Methode(n): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_115774
• UniProt-Hinterlegungsnummer: Q91VC4
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PLVAP(83483) ; mouse ... Plvap(84094)

Beschreibung

Allgemeine Beschreibung

Plasmalemma vesicle-associated protein (UniProt: Q91VC4; also known as MECA-32 antigen, Plasmalemma vesicle protein 1, PV-1) is encoded by the Plvap (also known as Pv1) gene (Gene ID: 84094) in murine species. MECA-32 is a single-pass type II membrane protein that is involved in the formation of stomatal and fenestral diaphragms of caveolae and may also function in microvascular permeability. It is expressed in lung, kidney, spleen, heart, muscle, eye, pancreas, thyroid, thymus, submaxillary gland, prostate, epididymis, uterus, and liver. MECA-32 contains three coiled coil regions (aa 140-160; 189-224; and 281-383). It has a short cytoplasmic domain (aa 1-26), a transmembrane domain (aa 27-47), and a long extracellular domain (aa 48-438). In mouse embryo it is expressed at 7 days post coitum (dpc). Its expression in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier. Between 12 to 16 dpc higher expression is observed in endothelial cell surfaces of blood vessels in the brain. (Ref.: Hallmann, R., et al. (1995). Dev. Dynamics. 202; 525-332).

Spezifität

Clone MECA-32 is a rat monoclonal antibody that detects Plasmalemma vesicle-associated protein (PLVAP) in murine species.

Immunogen

Mouse lymph node stromal cells.

Anwendung

Research Category
Entzündung & Immunologie

Anti-PLVAP, clone MECA-32, Cat. No. MABF2071, is a rat monoclonal antibody tht detects Plasmalemma vesicle-associated protein (PV_1) and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected PLVAP in Immunohistochemistry applications (Tanneau, G.M., et. al. (1999). J Histochem Cytochem. 47(12):1581-92; Hallmann, R., et. al. (1995). Dev Dyn. 202(4):325-32).

Immunohistochemistry Analysis: A representative lot detected PLVAP in Immunohistochemistry applications (Kopp, H.G., et. al. (2005). Blood. 106(2):505-13).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PLVAP in mouse liver tissue.

Immunoprecipitation Analysis: A representative lot detected PLVAP in Immunoprecipitation applications (Hallmann, R., et. al. (1995). Dev Dyn. 202(4):325-32).

Western Blotting Analysis: A representative lot detected PLVAP in Western Blotting applications (Hallmann, R., et. al. (1995). Dev Dyn. 202(4):325-32).

Qualität

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PLVAP in mouse kidney tissue lysate.

Zielbeschreibung

50- 55 kDa observed; 49.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physikalische Form

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.