MABF2045

Anti-Siglec-H Antibody, clone 551

clone 551, from rat

• Synonym(e): Sialic acid binding Ig-like lectin H, Siglech
• UNSPSC-Code: 12352203
• eCl@ss: 32160702

Eigenschaften

• Biologische Quelle: rat
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 551, monoclonal
• Speziesreaktivität: mouse
• Verpackung: antibody small pack of 25 μg
• Methode(n): flow cytometry: suitable; immunohistochemistry: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_848821.2
• UniProt-Hinterlegungsnummer: Q3Y597
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Siglech(233274)

Beschreibung

Allgemeine Beschreibung

Siglec-H (UniProt: Q3Y597; also known as Sialic acid binding Ig-like lectin H) is encoded by the Siglech gene (Gene ID: 233274) in murine species. Siglecs are sialic acid-binding immunoglobulin-like lectins that are expressed mostly in the immune system and may be involved in both adhesive and signaling functions. Siglec-H is a type I, CD33-related siglec-like transmembrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off in the mature form. The mature form has to ig-like domains (aa 32-141 and 151-234) in its extracellular region. Unlike other CD33-related siglecs, it lacks tyrosine-based signaling motifs in its cytoplasmic tail. Siglec-H is shown to be expressed on plasmaocytoid dendritic cells (pDCs) and can serve as a reliable marker for these cells. Siglec-H is also reported to serve as an endocytic receptor expressed on murine pDCs. Action of Siglec-H depends on a 12 kDa DNAX-activating protein (DAP). (Ref.: Zhang, J., et al. (2006). Blood 107(9); 3600-3608; Yun, TJ., et al., (2016), Cell Metabolism 23(5); 852-866).

Spezifität

Clone 551 is a rat monoclonal antibody that detects murine Siglec-H. It targets an epitope with in the extracellular domain.

Immunogen

Bone marrow-derived Type 1 interferon-producing cells.

Epitope: extracellular domain

Anwendung

Research Category
Entzündung & Immunologie

Anti-Siglec-H, clone 551, Cat. No. MABF2045, is a rat monoclonal antibody that detects Sialic acid binding Ig-like lectin H and has been tested for use in Flow Cytometry and Immunohistochemistry.

Immunohistochemistry Analysis: A representative lot detected Siglec-H in Immunohistochemistry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).

Flow Cytometry Analysis: A representative lot detected Siglec-H in Flow Cytometry applications (Yun, T.J., et. al. (2016). Cell Metab. 23(5):852-66).

Qualität

Evaluated by Immunohistochemistry in frozen mouse spleen tissue sections.

Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected Siglec-H in frozen mouse spleen tissue sections.

Zielbeschreibung

34.35 kDa calculated.

Physikalische Form

Protein G purified

Format: Purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable