MABF112

Anti-CD70 Antibody, clone TAN 1-7

clone TAN 1-7, from rat

• Synonym(e): CD70 antigen, CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7, CD70
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: TAN 1-7, monoclonal
• Speziesreaktivität: mouse
• Methode(n): flow cytometry: suitable; western blot: suitable
• Isotyp: IgG2aκ
• NCBI-Hinterlegungsnummer: NP_035747
• UniProt-Hinterlegungsnummer: O55237
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... Cd70(21948)

Beschreibung

Allgemeine Beschreibung

CD70 is an interesting membrane bound cellular factor that acts as a cytokine which binds the receptor CD27. Binding induces the proliferation and co-stimulation of T cells and enhances the generation of cytolytic T-cells. Thus, CD70 plays important roles in T cell adaptive T cell response as well as in innate immunity and is required for the proper activation of the anti-viral adaptive response. CD70 also serves as a regulatory role in early cytokine responses to pathogens through the maintenance of Treg cell survival and function. Recent research also indicates that CD70 expression could be important in autoimmune disease regulation and in treatment of autoimmune diseases such as rheumatoid arthritis. CD70 is expressed on T cells, splenocytes, and thymocytes and it belongs to the TNF family of cellular signaling proteins.

Immunogen

Epitope: Extracellular domain

Recombinant protein corresponding to the extracellular domain of mouse CD70.

Anwendung

Research Category
Entzündung & Immunologie

Research Sub Category
Immunoglobuline & Immunologie

Anti-CD70 Antibody, clone TAN 1-7 | MABF112 is an antibody against CD70 Antibody for use in Western Blotting, Flow Cytometry.

Flow Cytometry Analysis: 2 µg of this antibody detected CD70 in mouse bone marrow dendritic cells treated with 2000U/mL GM-CSF and 0.1 ug/mL of LPS.
Functional Blocking Analysis: A representative lot detected CD70 in splenic CD8a+ DCs (Arora, P., et al. (2014). Immunity. 40:105-116).

Qualität

Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CD70 in 10 µg of mouse spleen tissue lysate.

Zielbeschreibung

~27 kDa observed

Physikalische Form

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1