MAB5234
Anti-Neurocan Antibody
Chemicon®, from mouse
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About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
mouse
Qualitätsniveau
Antikörperform
purified immunoglobulin
Antikörper-Produkttyp
primary antibodies
Klon
monoclonal
Speziesreaktivität
chicken
Hersteller/Markenname
Chemicon®
Methode(n)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotyp
IgG1
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... NCAN(1463)
Allgemeine Beschreibung
Neurocan is the major soluble chondroitin sulfate proteoglycan in the brain. It is thought to play a functional role in axonal growth and guidance and in the establishment of specific neural pathways during embryonic brain development. Neurocan expression in the brain is developmentally regulated. Early on the major form of neurocan consists of a 245 kD core protein with approximately two chondroitin sulfate glycosoaminoglycan chains of 22 kD each. Later neurocan comprises a 180 kD core protein. Both forms of neurocan contain only chondroitin 4-sulfate glycosoaminoglycan chains. By virtue of their high expression at sites of neurnal damage and trauma, chondroitin sulfate proteoglycans, including neurocan, are thought to inhibit successful nerve regeneration.
Spezifität
Neurocan, chicken. Recognizes the middle region of neurocan.
Anwendung
Anti-Neurocan Antibody is an antibody against Neurocan for use in ELISA, IP & WB.
Western blot: <0.5 μg/mL
Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan.
Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.
Immunoprecipitation: 1 μg/mL
ELISA: 1 μg/mL, excellent for core protein, good for monomer
Immunocytochemistry: not tested
Immunohistochemistry: does not work on fixed samples, unfixed has not been tested.
Optimal working dilutions must be determined by the end user.
Western blot using anti-chick Neurocan (MAB5234). Samples are 1) Untreated embryonic chick brain extract, 2) chondroitinase-treated embryonic chick brain extract, 3) GST fusion proteins from the middle region of chick neurocan.
Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.
Immunoprecipitation: 1 μg/mL
ELISA: 1 μg/mL, excellent for core protein, good for monomer
Immunocytochemistry: not tested
Immunohistochemistry: does not work on fixed samples, unfixed has not been tested.
Optimal working dilutions must be determined by the end user.
Physikalische Form
Format: Purified
Sonstige Hinweise
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 2
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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