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Merck

MAB5232

Sigma-Aldrich

Anti-Presenilin-1 Antibody, loop, a.a. 263-378, CT, clone PS1-loop

ascites fluid, clone PS1-loop, Chemicon®

Synonym(e):

Anti-ACNINV3, Anti-AD3, Anti-FAD, Anti-PS1, Anti-S182

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

PS1-loop, monoclonal

Speziesreaktivität

rat, primate, mouse, human

Hersteller/Markenname

Chemicon®

Methode(n)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

Isotyp

IgG1

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... PSEN1(5663)

Allgemeine Beschreibung

Presenilin is a probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Presenilin requires the other members of the gamma-secretase complex to have a protease activity. It may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. The gamma-secretase complex stimulates cell-cell adhesion through its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, it cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. Futher researcher may implicate the complex in hematopoiesis. Defects in PSEN1 have been implicated in early-onset Alzheimer disease type 3 (AD3) and late-onset Alzheimer disease (LOAD).

Spezifität

Presenilin-1, loop

Immunogen

Epitope: C-terminus
Presenilin-1, loop fusion protein (amino acids 263-378), human

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Neurodegenerative Krankheiten
Anti-Presenilin-1 Antibody, loop, a.a. 263-378, C-terminus, clone PS1-loop detects level of Presenilin-1 & has been published & validated for use in IC, IH(P), IP & WB.
Immunocytochemistry:
A previous lot of this antibody was used in IC.Acetone or methanol fixed cells; or lightly fixed 2.5-3% PFA (5-10′ RT).

Immunohistochemistry:
A previous lot was used on 3% paraformaldehyde fixed tissue. Works on paraffin embedded tissue sections if fixed with PLP, acetone or other light fixations; 10% formalin fixation is untested.

Immunoprecipitation:
A previous lot of this antibody was used in IP.

Western blot:
Recognizes a protein of approximately 18-20 kD, and occasionally there is a doublet (the top band of which is phosphorylated).

Optimal working dilutions must be determined by the end user

Qualität

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected PRESENILIN-1 on 10 μg of PC12 lysates.

Zielbeschreibung

18-20 kDa

Physikalische Form

Unpurified
Mouse monoclonal IgG1 ascites with no preservatives.

Lagerung und Haltbarkeit

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
Brain tissue

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Empfehlung

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Structure of the catalytic pore of gamma-secretase probed by the accessibility of substituted cysteines.
Sato, C; Morohashi, Y; Tomita, T; Iwatsubo, T
The Journal of Neuroscience null
Mammalian APH-1 interacts with presenilin and nicastrin and is required for intramembrane proteolysis of amyloid-beta precursor protein and Notch.
Lee, SF; Shah, S; Li, H; Yu, C; Han, W; Yu, G
The Journal of Biological Chemistry null
Presenilin-1 regulates induction of hypoxia inducible factor-1?: altered activation by a mutation associated with familial Alzheimer's disease.
De Gasperi, R; Sosa, MA; Dracheva, S; Elder, GA
Mol. Neurodegener. null
gamma-Secretase dependent production of intracellular domains is reduced in adult compared to embryonic rat brain membranes.
Fr?nberg, J; Karlstrom, H; Winblad, B; Tjernberg, LO; Frykman, S
Testing null
Mark R Farmery et al.
The Journal of biological chemistry, 278(27), 24277-24284 (2003-04-17)
One characteristic feature of Alzheimer's disease is the deposition of amyloid beta-peptide (Abeta) as amyloid plaques within specific regions of the human brain. Abeta is derived from the amyloid beta-peptide precursor protein (beta-APP) by the intramembranous cleavage activity of gamma-secretase.

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