MAB353A4

Anti-Nestin Antibody, clone rat-401, Alexa Fluor^™ 488 conjugate

clone rat-401, from mouse, ALEXA FLUOR^™ 488

• Synonym(e): rat401, NES, Nbla00170, FLJ21841
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Konjugat: ALEXA FLUOR^™ 488
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: rat-401, monoclonal
• Speziesreaktivität: rat, mouse
• Darf nicht reagieren mit: human
• Methode(n): immunocytochemistry: suitable
• Isotyp: IgG1
• NCBI-Hinterlegungsnummer: NP_037119
• UniProt-Hinterlegungsnummer: P21263
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... NES(10763) ; mouse ... Nes(18008) ; rat ... Nes(25491)

Beschreibung

Allgemeine Beschreibung

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

Spezifität

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

Immunogen

Embryonic rat spinal cord

Anwendung

Research Category
Stammzellforschung

Neurowissenschaft

Research Sub Category
Neurofilament & Neuronen-Stoffwechsel

Neuronen- & Gliamarker

Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).

Qualität

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

Zielbeschreibung

The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa

Physikalische Form

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Lagerung und Haltbarkeit

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Hinweis zur Analyse

Control
Mouse cortical neural stem cells (SCR029)

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

ALEXA FLUOR is a trademark of Life Technologies

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable