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Key Documents

MAB328

Sigma-Aldrich

Anti-Oligodendrocytes Antibody, clone CE-1

ascites fluid, clone CE-1, Chemicon®

Synonym(e):

MOSP

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

CE-1, monoclonal

Speziesreaktivität

rat, mouse, chicken, monkey, feline, human

Hersteller/Markenname

Chemicon®

Methode(n)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable

Isotyp

IgM

Eignung

not suitable for Western blot

Versandbedingung

dry ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... OLIG2(10215)

Spezifität

In neonatal rat glia MAB328 stains oligodendrocytes and does not react with type 1 astrocytes, type 2 astrocytes or fibroblasts. Does not stain cultures of rat Schwann cells. In tissue sections MAB328 labels oligodendrocytes and CNS myelin with no labeling of peripheral myelin. MAB328 detects MOSP which is expressed at day 4-5 of neonatal rat, which is about 1-2 after the appearance of GC or sulfatide. The initial expression of MOSP occurs at the stage in development when oligodendrocytes are elaborating processes and just beginning to form membrane sheets (Mu QQ & C Dyer, 1994).

Immunogen

Rat glial membranes and whole brain white matter.

Anwendung

Research Category
Neurowissenschaft
Research Sub Category
Neuronen- & Gliamarker
Immunocytochemistry

Immunohistochemistry at 1:1,000-1:5000

Not suggested for use in Western blot.

Optimal working dilutions must be determined by end user.

IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB328

This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).
This Anti-Oligodendrocytes Antibody, clone CE-1 is validated for use in IP, IC, IH, IH(P) for the detection of Oligodendrocytes.

Lagerung und Haltbarkeit

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Myelin/oligodendrocyte-specific protein: a novel surface membrane protein that associates with microtubules.
Dyer, C A, et al.
Journal of Neuroscience Research, 28, 607-613 (1991)
Pluripotent stem cells engrafted into the normal or lesioned adult rat spinal cord are restricted to a glial lineage.
Q L Cao, Y P Zhang, R M Howard, W M Walters, P Tsoulfas, S R Whittemore
Experimental neurology null
Global forebrain ischemia results in differential cellular expression of interleukin-1beta (IL-1beta) and its receptor at mRNA and protein level.
Sairanen, TR; Lindsberg, PJ; Brenner, M; Siren, AL
Journal of Cerebral Blood Flow and Metabolism null
Rho activation patterns after spinal cord injury and the role of activated Rho in apoptosis in the central nervous system.
Dubreuil, CI; Winton, MJ; McKerracher, L
The Journal of cell biology null
C5b-9 complement complex in autoimmune demyelination: dual role in neuroinflammation and neuroprotection.
Horea Rus, Cornelia Cudrici, Florin Niculescu
Advances in Experimental Medicine and Biology null

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