MAB2575
Anti-Integrin α5β1 Antibody, clone BMC5
ascites fluid, clone BMC5, Chemicon®
Synonym(e):
VLA-5
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Alle Fotos(1)
About This Item
UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Empfohlene Produkte
Biologische Quelle
rat
Qualitätsniveau
Antikörperform
ascites fluid
Antikörper-Produkttyp
primary antibodies
Klon
BMC5, monoclonal
Speziesreaktivität
mouse
Hersteller/Markenname
Chemicon®
Methode(n)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Isotyp
IgG2b
NCBI-Hinterlegungsnummer
UniProt-Hinterlegungsnummer
Versandbedingung
dry ice
Posttranslationale Modifikation Target
unmodified
Angaben zum Gen
human ... ITGA5(3678)
Spezifität
Antibody MAB2575 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.
Immunogen
Mouse embryonal carcinoma P19 cells.
Anwendung
Research Category
Zellstruktur
Zellstruktur
Research Sub Category
Integrine
Integrine
Detect Integrin α5β1 using this Anti-Integrin α5β1 Antibody, clone BMC5 validated for use in FC, IP, IH.
Inhibition of cell adhesion to fibronectin: 10 μL/mL
Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.
Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μL
Flow cytometry: 10μL/mL
Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.
Optimal working dilutions must be determined by end user.
Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete.
Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μL
Flow cytometry: 10μL/mL
Immunohistochemistry on acetone-fixed frozen sections only; formalin fixation is not recommended.
Optimal working dilutions must be determined by end user.
Physikalische Form
Liquid ascites.
Lagerung und Haltbarkeit
Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
Rechtliche Hinweise
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Lagerklassenschlüssel
10 - Combustible liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Invasion of eukaryotic cells by Borrelia burgdorferi requires ?(1) integrins and Src kinase activity.
Wu, J; Weening, EH; Faske, JB; Hook, M; Skare, JT
Infection and Immunity null
Integrin adhesion and force coupling are independently regulated by localized PtdIns(4,5)2 synthesis.
Legate, KR; Takahashi, S; Bonakdar, N; Fabry, B; Boettiger, D; Zent, R; Fassler, R
The Embo Journal null
Jessie Zhong et al.
PloS one, 7(4), e35058-e35058 (2012-04-18)
The speed of cell migration on 2-dimensional (2D) surfaces is determined by the rate of assembly and disassembly of clustered integrin receptors known as focal adhesions. Different modes of cell migration that have been described in 3D environments are distinguished
Mitasha Bharadwaj et al.
Nature communications, 8, 14348-14348 (2017-01-28)
Upon binding to the extracellular matrix protein, fibronectin, αV-class and α5β1 integrins trigger the recruitment of large protein assemblies and strengthen cell adhesion. Both integrin classes have been functionally specified, however their specific roles in immediate phases of cell attachment
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