MAB2053

Anti-Disialoganglioside GD3 Antibody, clone MB3.6

clone MB3.6, Chemicon^®, from mouse

• Synonym(e): GD3 Antibody
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: MB3.6, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Chemicon^®
• Methode(n): flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable
• Isotyp: IgG3
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Spezifität

Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells.

Immunogen

FM9 melanoma cells

Anwendung

Anti-Disialoganglioside GD3 Antibody, clone MB3.6 is an antibody against Disialoganglioside GD3 for use in FC, IF, IH.

Immunohistochemistry (frozen tissue sections)

Immunofluorescence of cells in culture

Flow cytometry: 1-2 μg per 10E6 cells

Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).

Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572.

TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}





Optimal working dilutions must be determined by end user.

Physikalische Form

Format: Purified

Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing 0.1% NaN3.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable