MAB1678

Anti-Filamin A Antibody, clone PM6/317

ascites fluid, clone PM6/317, Chemicon^®

• Synonym(e): Alpha-Filamin, Filamin I, Endothelial Actin-binding Protein, ABP-280, Nonmuscle Filamin
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: ascites fluid
• Antikörper-Produkttyp: primary antibodies
• Klon: PM6/317, monoclonal
• Speziesreaktivität: human, rabbit, rat, guinea pig, chicken, mouse
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG1
• NCBI-Hinterlegungsnummer: NM_001110556.1
• UniProt-Hinterlegungsnummer: P21333
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FLNA(2316) ; mouse ... Flna(192176)

Beschreibung

Allgemeine Beschreibung

Filamin is a structural protein that forms flexible cross-links between two actin filaments. Filamin is a homodimer of polypeptide chains each joined to the other at one end with an actin binding site ath the other. It is present in smooth muscle, fibroblasts, platelets and lymphocytes.

Spezifität

Expected to cross-react with chicken, guinea pig and rabbit.

Recognizes unprocessed, full length Human filamin (actin-binding protein; 270-280 kDa) as well as the 190 kDa N-terminal calpain cleavage fragment of filamin (Aakhus, 1992). Following induction of apoptosis in U937 cells, the antibody recognizes 170, 150, and 120 kDa N-terminal cleavage fragments of the full-length form presumably resulting from cleavage by activated caspase-3 (Umeda, 2001).

Immunogen

Human platelet protein.

Anwendung

Research Category
Zellstruktur

Research Sub Category
Cytoskelett

Immunoblotting:
1:1000-1:4000. Because of the large size of the unprocessed forms of filamin, 4-7% PAGE gels and proteinase inhibitors are recommended.

Immunofluorescence:
1:50-1:200 dilution from a previous lot was used. Suitable for staining both frozen and paraffin embedded tissues (at lower dilutions). Microwave-citrate buffer antigen retrieval method recommended for paraffin sections.

Immunoprecipitation:
A previous lot was used on immunoprecipitation. Suggested lysis buffer is PBS with 0.5% triton X-100 with proteinase inhibitors (note for full length filamin include calpain inhibitors). 5 microliters of antibody for every 300-500 μL of cell lysate (200-500 μg/mL total protein is suggested. Incubation is 1 hour RT or overnight 4C; Protein A/G agarose beads or rabbit anti-mouse secondary capture antibody is recommended for best recovery. 4-8% acrylamide gels are recommended for full length filamin or the 190 kDa fragement visualization.

Optimal working dilutions must be determined by end user.

This Anti-Filamin A Antibody, clone PM6/317 is validated for use in IF, IH, IH(P), IP, WB for the detection of Filamin A.

Qualität

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis:
1:500-1:4000 dilution of this lot detected Filamin A on 10 μg of Jurkat lysates. Because of the large size of the unprocessed forms of filamin, 4-7% PAGE gels and proteinase inhibitors are recommended.

Zielbeschreibung

270-280 kDa & 190 kDa

Physikalische Form

Unpurified

Mouse monoclonal ascites IgG1 in buffer containing 0.1% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Hinweis zur Analyse

Control
Positive control tisse: skin, jurkat cell lysate.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: nwg
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable