MAB1285FX

Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated

clone HLA.ABC.m3, Chemicon^®, from mouse

• Synonym(e): Anti-AS, Anti-HLAB
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100; 300
• Konjugat: FITC conjugate
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: HLA.ABC.m3, monoclonal
• Speziesreaktivität: human
• Hersteller/Markenname: Chemicon^®
• Methode(n): flow cytometry: suitable
• Isotyp: IgG2a
• NCBI-Hinterlegungsnummer: NM_005514.5
• UniProt-Hinterlegungsnummer: P01889
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... HLA-B(3106)

Beschreibung

Allgemeine Beschreibung

BIOCHEMISTRY: pI = 8.0

The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.

Spezifität

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x10⁸M-1) than for HLA-B7 (9.5x10⁷M-).

Immunogen

Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA antibody and staphylococcal Protein A.

Anwendung

Research Category
Entzündung & Immunologie

Research Sub Category
Immunoglobuline & Immunologie

Detect HLA-B27 using this Anti-HLA-B27 Antibody, clone HLA.ABC.m3, FITC conjugated validated for use in FC.

This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

Flow cytometry and immunofluorescence 1:15 to 1:60

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

Physikalische Form

Purified from mouse ascitic fluid. Fluorescein conjugated. The antibody is supplied in PBS 0.01M, Sodium Azide 0.1%, pH 7.1, 0.2% BSA. The characteristics of each lot are tested by flow cytometry.

Lagerung und Haltbarkeit

Store at 2 to 8°C. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable