MAB10764

Anti-SBP-tag Antibody, clone 20 | MAB10764

clone 20, 1.0 mg/mL, from mouse

• Synonym(e): streptavidin binding peptide
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 20, monoclonal
• Speziesreaktivität: human
• Konzentration: 1.0 mg/mL
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG1κ
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified

Beschreibung

Allgemeine Beschreibung

Streptavidin Binding Peptide tag is a useful and versatile peptide that can be inserted into expression vectors for easy detection of recombinant proteins and purification. The sequence for SBP-tag is 38 amino acids long having a nanomolar scale binding affinity for streptavidin with an equilibrium constant of 2.5 nM.

Spezifität

Demonstrated to react with cells expressing the SBP tag.

This antibody recognizes SBP tag.

Immunogen

Epitope: Unknown

Linear peptide corresponding to SBP tag.

Anwendung

Research Category
Epitop-Tags & allgemeine Anwendung

Research Sub Category
Epitop-Tags

Immunofluorescence Analysis: A previous lot was used by an independent laboratory in IF. (Hudson, D., et al. (2008). MBC. 19(7):3070-3079.)

Qualität

Evaluated by Western Blot in pTrAP-GFP transfected Hek293 lysate.

Western Blot Analysis: 0.1 ng/mL of this antibody detected SBP-tag on 10 µg of pTrAP-GFP transfected Hek293 lysate.

Zielbeschreibung

The protein expresss is pTrAP-GFP plasmids containing a Histidine tag, S-tag, streptavidin-binding pepide (SBP) tag, and GFP. The protein expressing is ~37 kDa

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
pTrAP-GFP transfected Hek293 lysate

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable