CBL440

Anti-c-Fos Antibody

CHEMICON^®, sheep polyclonal

• Synonym(e): G0/G1 Switch Regulatory Protein 7
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• product name: Anti-c-Fos Antibody, Chemicon^®, from sheep
• Biologische Quelle: sheep
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: human, rat
• Hersteller/Markenname: Chemicon^®
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NM_005252.2
• UniProt-Hinterlegungsnummer: P01100
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... FOS(2353)

Beschreibung

Spezifität

c-fos is a 55 kDa nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents. c-fos is also induced in neuronal tissue following drug induced seizures.

KNOWN SPECIES CROSS REACTIVITY: Known to cross-react with rat

Immunogen

Synthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala-Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
Transkriptionsfaktoren

Anti-c-Fos Antibody is a Sheep Polyclonal Antibody for detection of c-Fos also known as G0/G1 Switch Regulatory Protein 7 & has been validated in WB, ICC.

Immunoblotting: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein. CBL 440 detects a band at 55-60 kDa on immunoblots of stimulated HeLa and HL-60 lysates.

Immunocytochemistry: CBL 440 has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (where CBL 440 detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment.

Optimal working dilutions must be determined by the end user.

Physikalische Form

Format: Purified

The purified immunoglobulin is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Lagerung und Haltbarkeit

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable